Overview

  • Product name
  • Description
    Rabbit polyclonal to BrdU
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Chemical/ Small Molecule corresponding to BrdU.

  • Positive control
    • HeLa cells treated with 100µM BrdU for 4hrs.
  • General notes

    This antibody has so far been only tested in cultured cells (ICC/IF), but not with tissue sections (IHC).

Properties

Applications

Our Abpromise guarantee covers the use of ab152095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.
IHC-P Use at an assay dependent concentration. PubMed: 28397811

Target

  • Relevance
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization
    Nuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells treated with or without 100µM BrdU for 4 hours, labeling BrdU with ab152095 at 1/50 dilution. Lower images stained with Hoechst 33342.

References

This product has been referenced in:
  • Seth B  et al. Carbofuran hampers oligodendrocytes development leading to impaired myelination in the hippocampus of rat brain. Neurotoxicology 70:161-179 (2019). Read more (PubMed: 30471306) »
  • Tang X  et al. The role of the fat mass and obesity-associated protein in the proliferation of pancreatic cancer cells. Oncol Lett 17:2473-2478 (2019). Read more (PubMed: 30719115) »
See all 16 Publications for this product

Customer reviews and Q&As

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1-4 of 4 Abreviews

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Resin sections)
Sample
Rabbit Tissue sections (Brain (agar sections))
Specification
Brain (agar sections)

Dr. Patricia Sampedro-Piquero

Verified customer

Submitted Jan 09 2019

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
Yes - 0,25% Triton X-100
Specification
Brain
Fixative
Paraformaldehyde

Mr. Manuel Narvaez

Verified customer

Submitted Oct 16 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse brain)
Antigen retrieval step
Other
Permeabilization
Yes - 0.3% Triton X-100
Specification
mouse brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Paraformaldehyde

Jacob Garza

Verified customer

Submitted Sep 14 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3 % triton
Fixative
Paraformaldehyde

Dr. Sébastien Brot

Verified customer

Submitted Oct 29 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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