Question (37196) | Anti-BrdU antibody (Biotin) (ab2284)

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Question

We want to use the biotinylated anti-BrdU antibody without a secondary antibody. I was wondering if you would suggest blocking the tissue with hydrogen peroxide or denaturing the DNA with HCl first?

Answer

You will denature the DNA first and then carry on with the IHC staining protocol.

https://www.abcam.com/index.html?pageconfig=resource&rid=11533
https://www.abcam.com/assets/popups/popup_datasheet_protocols_printer_friendly.cfm?pid=1325&intAbId=2284

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