Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BrdU antibody (Biotin)

Excellent
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Cell (free floating Brain slices)
Specification
free floating Brain slices
Fixative
Paraformaldehyde
Antigen retrieval step
None
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10

Other product details

Dilution
1/2000
Incubation time
24 hour(s) and 0 minute(s)

Secondary antibody

Secondary antibody
None used

Additional data

Additional Notes
The image depicts the subventricular zone.
A strpetavidin-HRP complex and DAB were used for detection.
Immunohistochemistry was performed on free floating 40 µm vibratome sections obtained from paraformaldehyde fixed brains

Dr. Christoph Schwarzer

Verified customer

Submitted Nov 03 2006

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