Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody (Biotin)

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (brain,lateral ventricle)
Specification
brain,lateral ventricle
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Tris Buffered Saline- 0.3% Triton X
Permeabilization
Yes - 4N HCl
Blocking step
NDS as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Dilution
1/1000
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: Tris Buffered Saline and 0,3% Triton X

Secondary antibody

Name
Non-Abcam antibody was used: anti sheep-Vector/Szabo
Host species: Donkey
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
Typical Brdu positive DAB-staining of mouse brain, subventricular zone of lateral ventricle 10X magnification.In the right upper part, a magnification of these Brdu positive cells that are going to migrate in the rostra migratory stream (RSM) to the olfactory bulb and differentiate into neurons and glia.

Mr. stefano gaburro

Verified customer

Submitted Nov 01 2007

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