Immunohistochemistry (Frozen sections) abreview for Anti-BrdU antibody [BU1/75 (ICR1)]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain Section)
Specification
Brain Section
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Other product details

Dilution
1/200
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488 Goat anti-Rat IgG (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Before permeabilization, pretreatment is performed: incubate with 2N HCl at RT for 30 min and wash 3 times. BrdU staining is shown in green and NeuN staining is shown in red.

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Submitted Feb 26 2010

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