Immunohistochemistry (Frozen sections) abreview for Anti-BrdU antibody [BU1/75 (ICR1)]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (colon)
Permeabilization
Yes - 0.1% Triton X-100
Specification
colon
Blocking step
Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA
Dilution
1/200

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Goat anti-Rat Alexa Fluor 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594

Additional data

Additional Notes
Heat-mediated antigen retrieval significantly improves staining.

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Submitted May 24 2018

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