Immunohistochemistry free floating abreview for Anti-BrdU antibody [BU1/75 (ICR1)]

Excellent
Abreviews
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Hippocampus (brain), 45 µm VIBRATOME sections)
Specification
Hippocampus (brain), 45 µm VIBRATOME sections

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS/Triton with donkey serum
Dilution
1/200

Secondary antibody

Dilution
1/500
Name
Non-Abcam antibody was used: Rabbit anti-Rat IgG (H+L) Secondary Antibody, Biot
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin

Additional data

Additional Notes
“IHC-vibratome sections” Fixative: Paraformaldehide Antigen retrieval (Target Retrieval Solution, High pH, DAKO (1:50) 1 min in microwave) Permeabilization: 2N HCl at 37ºC 30 minutes followed by 10 min in borate buffer

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Submitted Mar 04 2019

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