Immunocytochemistry/ Immunofluorescence abreview for Anti-BrdU antibody [BU1/75 (ICR1)]

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hiPSC)
Permeabilization
No
Specification
hiPSC
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
carnoys

Other product details

Incubation time
1 hour(s) and 0 minute(s)
Dilution
1/400

Secondary antibody

Dilution
1/250
Name
Non-Abcam antibody was used: goat anti rat 555 cross absorbed
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 555

Additional data

Additional Notes
Images were taken using a confocal microscope. In red DNA fibres labelled with IdU and detected with anti-BrdU (ab6326) from abcam. In green DNA fibres labelled CldU and stained using a non-abcam mouse anti-BrdU antibody.

Abcam user community

Verified customer

Submitted May 14 2019

Sign up