For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    brdu-antibody-bu175-icr1-proliferation-marker-ab6326.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cell Biology Cell Cycle Markers
Share by email

Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

  • Datasheet
  • SDS
Reviews (97)Q&A (29)References (1165)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
  • Flow Cytometry - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
  • Flow Cytometry - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

Key features and details

  • Rat monoclonal [BU1/75 (ICR1)] to BrdU - Proliferation Marker
  • Suitable for: ICC/IF, IHC-P, Flow Cyt
  • Reacts with: Species independent
  • Isotype: IgG2a

You may also be interested in

Primary
Product image
Anti-MCM2 (phospho S108) antibody [EPR4121] (ab109271)
Biochemical
Product image
BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
Primary
Product image
Anti-IdU antibody [OTI2F8] (ab187742)

View more associated products

Overview

  • Product name

    Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker
    See all BrdU primary antibodies
  • Description

    Rat monoclonal [BU1/75 (ICR1)] to BrdU - Proliferation Marker
  • Host species

    Rat
  • Tested applications

    Suitable for: ICC/IF, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • Positive control

    • ICC/IF: HeLa cells; Flow Cyt: HeLa cells; IHC-P: Rat small intestine tissue.
  • General notes

    Protocol advice:

    This antibody recognizes single stranded DNA so the DNA needs to be unraveled first. This can be done with DNAse, although it doesn't give the best results. Depending on the assay, acid denaturation with 2M HCL or heat denaturation are the most successful. Please note this step is critical in any assay with this antibody and is the area that should be modified to optimize results. A detailed BrdU staining protocol is available in the Protocols tab or by clicking on this link.

    Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956. 

    This monoclonal antibody is manufactured exclusively by Abcam. 

    AF488 conjugate available as ab220074
    AF647 conjugate available as ab220075
    Please see the Associated Products tab for other available conjugates. 

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Some batches contain 6.97% L-Arginine as a stabilizing agent. For lot-specific buffer information, please contact our Scientific Support team.
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Monoclonal
  • Clone number

    BU1/75 (ICR1)
  • Isotype

    IgG2a
  • Light chain type

    kappa
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Tags & Cell Markers
    • Cell Type Markers
    • Replication
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220074)
    • Alexa Fluor® 647 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220075)
    • Alexa Fluor® 594 Anti-BrdU antibody [BU1/75 (ICR1)] (ab220076)
    • HRP Anti-BrdU antibody [BU1/75 (ICR1)] (ab220507)
    • Alexa Fluor® 555 Anti-BrdU antibody [BU1/75 (ICR1)] (ab221240)
    • Anti-BrdU antibody [BU1/75 (ICR1)] - BSA and Azide free (ab264079)
  • Compatible Secondaries

    • Goat Anti-Rat IgG H&L (HRP) (ab205720)
  • Isotype control

    • Rat IgG2a, kappa monoclonal [RTK2758] - Isotype Control (ab18450)
  • Related Products

    • BrdU Cell Proliferation ELISA Kit (colorimetric) (ab126556)
    • BrdU Cell Proliferation ELISA Kit (chemiluminescent) (ab126572)
    • BrdU (5-bromo-2'-deoxyuridine), Thymidine analog (ab142567)
    • Fix & Perm / Cell Fixation & Permeabilization Kit (Flow Cytometry) (ab185917)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab6326 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF (26)
1/250.
IHC-P (27)
Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt (2)
1/25 - 1/200.

Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free) (ab18450), is suitable for use as an isotype control with this antibody.

Notes
ICC/IF
1/250.
IHC-P
Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/25 - 1/200.

Rat IgG2a, kappa monoclonal [RTK2758] (Low endotoxin, Azide Free) (ab18450), is suitable for use as an isotype control with this antibody.

Target

  • Relevance

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization

    Nuclear
  • Alternative names

    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

    ab6326 stained in Hela cells. Untreated and BrdU treated (10uM for 24 hours) cells were fixed with 100% methanol (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then incubated with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% triton for 1h at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab6326 at 5µg/ml and ab7291 (Mouse monoclonal to alpha tubulin) at 1ug/ml overnight at +4°C. The secondary antibodies were Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) (colored green) used at 2 ug/ml and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) (pseudo-colored red) used at 1/1000 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Flow Cytometry - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Flow Cytometry - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

    Dot plot showing BrdU-treated HeLa cells stained with ab6326. Cells were incubated with 10 µM BrdU for 30 minutes prior to being harvested, washed twice in 1x PBS and fixed in 70% ethanol (4°C, added drop-wise) for at least 30 minutes on ice. Once fixed, pellets were acid denatured with 2M HCl for 30 minutes at 22ºC and then neutralised with borate buffer (0.1M, pH8.5).

    Samples were washed and incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab6326, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150165) at 1/2000 dilution for 30 min at 22ºC.

    7-AAD was added to cells 20 min prior to data acquisition.

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) with 530/30 and 685/35 bandpass filters.

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)Image from Condezo GN et al., PLoS Pathog. 2017;13(4):e1006320. Fig 1.; doi: 10.1371/journal.ppat.1006320. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Confocal immunofluorescence sections (~0.3 μm thick) showing the localization of the different factors in HEK 293 cells infected with Ad5 wt. Double labeling for BrdU (ab6326, magenta) and L1 52/55 kDa (green). Dotted rectangles indicate the areas shown at higher magnification in the bottom row. Bars: 3 μm.

    Monolayer HEK293 cells grown in cover glasses were infected with Ad5 wt. The infection was synchronized by incubating the cells for 30 min at 4°C and then 30 min at 37°C. Then, the inoculums were removed and medium was added. For BrdU labeling, after 18 h at 37°C the medium was changed by medium containing 25 μg/ml BrdU (5-Bromo-2’-deoxyuridine), followed by another change at 25 hpi. Incubation with BrdU proceeded at 37°C. After 36 hpi, the medium was removed and 4% paraformaldehyde in PBS was added to the cells during 10 min. After 3 rinses with PBS, cover glasses were incubated with a mixture of 0.5% saponin and 10% FBS in PBS for 10 min. Samples were incubated with the primary antibody in 0.5% saponin and 2% FBS in PBS during 45 min. After three more rinses, incubation with secondary antibodies diluted in 0.5% saponin and 2% FBS in PBS was carried out in darkness. After a final rinse with PBS, cover glasses were mounted on glass slides. Antifade reagent was allowed to dry overnight before sample observation. All incubations were carried out at room temperature. Images were taken using a confocal multispectral Leica TCS SP5 system.

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

    ICC/IF image of ab6326 stained HeLa cells, both BrdU treated (left image) and normal cells (right image). The cells were 100% methanol fixed (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6326, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rat IgG H&L (DyLight® 488) preadsorbed (ab98420) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Positive staining can be seen in the BrdU treated cells, but not in the normal cells, demonstrating specificity for BrdU.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

    IHC image of ab6326 staining in a formalin-fixed, paraffin-embedded rat small intestine BrdU tissue section. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins, and incubated overnight at +4°C with ab6326 at 3 ug/ml. A goat anti-rat biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. The section was counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) users should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)This image is courtesy of an anonymous Abreview

    ab6326 staining cultured cells of rat brain tissue by ICC.  The sample was PFA fixed and permeabilized in 1M HCl prior to blocking with 5% serum for 1 hour at 25°C.  The primary antibody was diluted 1/500 and incubated with the sample for 16 hours at 25°C.  A biotinylated rabbit anti-rat IgG antibody, diluted 1/200, was used as the secondary.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)Image from Vandenplas S et al., PLoS One. 2016;11(4):e0152870. Fig 2.; doi: 10.1371/journal.pone.0152870. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Immunohistological staining of frontal (A-C) and sagittal (D) sections through a tooth family on the lower jaw of S. salar with BrdU labelled cells in green and DAPI counterstain in blue. The tooth families presented are in a similar state of development: a young functional tooth and its successor in morphogenesis stage. (A) Pulsed specimen, T0; (B) Chase time T1, one week after BrdU administration; (C) Chase time T2, two weeks after BrdU administration and (D) Chase time T3, four weeks after BrdU administration. The white arrow indicates single cells within the middle dental epithelium (MDE) enclosed by the IDE, cervical loop and ODE of the replacement tooth. Abbreviations: Ab: aboral; Ant: anterior; CV: cervical loop; DP: dental papilla; FT: functional tooth; IDE: inner dental epithelium; Lab: labial; Lin: lingual; MDE: middle dental epithelium; ME: mesenchyme; OE: oral epithelium; ODE: outer dental epithelium; Or: oral; PC: pulp cavity; Pos: posterior; yellow asterisk: replacement tooth; scale bars: 100 μm.

    Immunohistological staining for BrdU on the paraffin sections: Rehydration through a decreasing ethanol series, chromatin precipitation in hydrochloric acid, block in 3% BSA/ 1% milk powder, exposure to primary antibody (ab6326) and secondary antibody (polyclonal anti-rat Alexa Fluor® 488), DAPI counterstaining (1μl/ml). Immunofluorescence was visualized on a NIKON eclips TE2000-S confocal laser-scanning microscope. Adobe Illustrator CS5, Adobe Photoshop CS5 and Fiji were used to process bright field and fluorescent images of the immunostained sections.

  • Flow Cytometry - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Flow Cytometry - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)This image is courtesy of an anonymous Abreview
    ab6326 staining BrdU in HeLa cells by Flow Cytometry. Cells were incubated with 10 µM BrdU for 30 minutes prior to being harvested with 1X trypsin-EDTA, washed twice in PBS containing 1% BSA, and fixed in 70% ethanol (added drop-wise) for at least 30 minutes on ice. Once fixed, pellets were acid denatured with HCl/Triton X-100 for 30 minutes at room temperature and then neutralised with sodium tetraborate.
    Pelleted cells were re-suspended in Tween/BSA/PBS to which primary antibody was then added (0.1 µg in 0.5% Tween 20 (v/v) plus 1% BSA in PBSA) and incubated for 30 minutes at room temperature. Secondary Alexa Fluor®488-conjugated Goat anti-Rat IgG (H+L) was used at 1/500 and incubated for 30 minutes at room temperature in the dark. Cells were pelleted once more and resuspended in PBS containing 5 µg/mL propidium iodide.
    Gating Strategy: Based on forward and side scatter, cells were gated into the region used for analysis. This was done by applying a large circle to a

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)This image is courtesy of an anonymous Abreview

    ab6326 staining BrdU in mouse lung tissue sections by Immunohistochemistry (Formalin/PFA-fixed parafin-embedded sections). Tissue samples were heat mediated with  Citrate buffer for 40min. The sections were blocked with 10% donkey serum for 1 hour at 22°C.  Samples were incubated with the primary antibody (1/200 in 10% Donkey serum-PBS 0.2% Triton) at 4°C for 16 hours. Cy™3 AffiniPure Donkey Anti-Rat IgG (H+L) (1/400) was used as the secondary antibody.

    Representative confocal images of terminal bronchioles in mouse lungs taken at day 5 following naphthalene injection.

    Colors label CC10 (red), FoxJ1 (green), BrdU (yellow), and nuclear DNA (blue).

    See Abreview

Protocols

  • BrdU staining protocol

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (1165)

    Publishing research using ab6326? Please let us know so that we can cite the reference in this datasheet.

    ab6326 has been referenced in 1165 publications.

    • Ramon-Duaso C  et al. Protective effects of mirtazapine in mice lacking the Mbnl2 gene in forebrain glutamatergic neurons: Relevance for myotonic dystrophy 1. Neuropharmacology N/A:108030 (2020). PubMed: 32171677
    • Saric N  et al. The AHR pathway represses TGFß-SMAD3 signalling and has a potent tumour suppressive role in SHH medulloblastoma. Sci Rep 10:148 (2020). PubMed: 31924815
    • Chen M  et al. CTNNB1/ß-catenin dysfunction contributes to adiposity by regulating the cross-talk of mature adipocytes and preadipocytes. Sci Adv 6:eaax9605 (2020). PubMed: 31934629
    • Bywater MJ  et al. Reactivation of Myc transcription in the mouse heart unlocks its proliferative capacity. Nat Commun 11:1827 (2020). PubMed: 32286286
    • Sun L  et al. Astragaloside IV Exerts Cognitive Benefits and Promotes Hippocampal Neurogenesis in Stroke Mice by Downregulating Interleukin-17 Expression via Wnt Pathway. Front Pharmacol 11:421 (2020). PubMed: 32317974
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

    Filter by Application

    Filter by Species

    Filter by Ratings

    1-10 of 97 Abreviews

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More

    Mr. Musaad Alshammari

    Verified customer

    Submitted Jan 11 2016

    Immunohistochemistry free floating abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry free floating
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 14 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (mouse brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Sodium citrate buffer
    Permeabilization
    Yes - 0.2% TritonX-100 in PBS for 3hours along with blocking solution.
    Specification
    mouse brain
    Blocking step
    Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 28°C
    Fixative
    Paraformaldehyde
    Read More

    Mr. Lenin Veeraval

    Verified customer

    Submitted Nov 06 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Primary Fibroblasts)
    Permeabilization
    Yes - 0.3% Triton in PBS 20 min
    Specification
    Human Primary Fibroblasts
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 21 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA Ph 9.0
    Permeabilization
    No
    Specification
    Liver
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 29 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Small intestine)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: BioCare Diva decloaker
    Permeabilization
    No
    Specification
    Small intestine
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 2.5% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Mr. Victor Bernal

    Verified customer

    Submitted Aug 28 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Skin fibroblasts)
    Permeabilization
    Yes - 2N HCl for 20 min and 0.3% triton X100 in PBS for 20 min
    Specification
    Skin fibroblasts
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 06 2020

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (MC3T3 cells)
    Permeabilization
    Yes - NP40
    Specification
    MC3T3 cells
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 16 2019

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Nothobranchius furzeri Tissue sections (brain)
    Antigen retrieval step
    Other
    Permeabilization
    Yes - TBS
    Specification
    brain
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Read More

    Jolien Van houcke

    Verified customer

    Submitted Jul 05 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (hiPSC)
    Permeabilization
    No
    Specification
    hiPSC
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Fixative
    carnoys
    Read More

    Abcam user community

    Verified customer

    Submitted May 14 2019

    1-10 of 97 Abreviews

    •  Previous
    • 1
    • 2
    • 3
    • 4
    • 5
    • ...
    • 10
    • Next 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.