Anti-BrdU antibody [Bu20a] (ab136650)

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Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [Bu20a] (ab136650)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [Bu20a] (ab136650)

Key features and details

  • Mouse monoclonal [Bu20a] to BrdU
  • Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-P
  • Reacts with: Species independent
  • Isotype: IgG1

Overview

  • Product name

    Anti-BrdU antibody [Bu20a]
    See all BrdU primary antibodies

  • Description

    Mouse monoclonal [Bu20a] to BrdU

  • Host species

    Mouse

  • Specificity

    ab136650 reacts specifically with BrdU incorporated into DNA during S phase of a cell cycle.

  • Tested applications

    Suitable for: IHC-Fr, ICC/IF, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    BrdU conjugated to BSA.

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab136650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration.

Target

  • Relevance

    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.

  • Cellular localization

    Nuclear

  • Alternative names

    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [Bu20a] (ab136650)
    Immunocytochemistry/ Immunofluorescence - Anti-BrdU antibody [Bu20a] (ab136650)

    ICC/IF image of ab136650 stained HeLa cells, both BrdU treated (left image) and normal cells (right image). The cells were 100% methanol fixed (5 min) and then subjected to acid hydrolysis using 2M HCL in 0.1% PBS-Tween for 30 minutes at room temperature to denature the DNA. They were then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab136650, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. Positive staining can be seen in the BrdU treated cells, but not in the normal cells, demonstrating specificity for BrdU.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [Bu20a] (ab136650)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BrdU antibody [Bu20a] (ab136650)

    IHC image of ab136650 staining, both in normal and BrdU treated rat liver formalin fixed paraffin embedded tissue sections, performed on a Leica Bond

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References (5)

Publishing research using ab136650? Please let us know so that we can cite the reference in this datasheet.

ab136650 has been referenced in 5 publications.

  • Heredia M  et al. Factors Involved in the Functional Motor Recovery of Rats with Cortical Ablation after GH and Rehabilitation Treatment: Cortical Cell Proliferation and Nestin and Actin Expression in the Striatum and Thalamus. Int J Mol Sci 20:N/A (2019). PubMed: 31744113
  • Giraddi RR  et al. Stem and progenitor cell division kinetics during postnatal mouse mammary gland development. Nat Commun 6:8487 (2015). PubMed: 26511661
  • Silverman BR & Champion JA Presentation of fibronectin fragments using affinity protein interactions for enhanced retention and function. Acta Biomater 10:4956-60 (2014). PubMed: 25173838
  • Calabrese C  et al. Cell proliferation of esophageal squamous epithelium in erosive and non-erosive reflux disease. World J Gastroenterol 17:4496-502 (2011). PubMed: 22110280
  • Magaud JP  et al. Double immunocytochemical labeling of cell and tissue samples with monoclonal anti-bromodeoxyuridine. J Histochem Cytochem 37:1517-27 (1989). ICC/IF ; Human, Mouse . PubMed: 2476478

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