Overview

  • Product name
    Anti-BrdU antibody (HRP)
    See all BrdU primary antibodies
  • Description
    Sheep polyclonal to BrdU (HRP)
  • Host species
    Sheep
  • Conjugation
    HRP
  • Specificity
    The antibody was tested using immunoprecipitation against 5-Methyl Cytosine (5-MeC) and bromo-deoxyuridine (BrdU) or control (no antigen) and assayed by A-405 spectrophotometry. At a concentration of 25 µg/mL, this product demonstrates 8 fold higher reactivity against BrdU than 5-MeC. This product titers out at 50 ng/mL. For best results, use the product at a concentration of 25 to 100 µg/mL. Nearly complete immunoprecipitation was obtained at a concentration of 100 to 500 µg/mL. This antibody is not species specific. The nucleotide sequence is present in all species.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: a wide range of other species
  • Immunogen

    Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)

Properties

Applications

Our Abpromise guarantee covers the use of ab2285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • Relevance
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization
    Nuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

References

This product has been referenced in:
  • Sano H  et al. Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper. Proc Natl Acad Sci U S A 77:3581-5 (1980). Read more (PubMed: 6251470) »

See 1 Publication for this product

Customer reviews and Q&As

Thank you for your inquiry. Yes, I can confirm that you are absolutely right, you will just need the DAB.

Indeed, HRP is the enzyme (horse radish peroxidase) which will convert the substrate (DAB) into the colored precipitation seen on the sl...

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The laboratory has used this antibody at a concentration of 10 µg/mL to obtain a good signal, comparable to other antibodies. As the antibody is at a concentration of 0.5mg/ml, this corresponds to a dilution of 1/50.

We do however recommend t...

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The actual protocols used can be found in the following publication. Campana D, Coustan-Smith E., Janossy G., Department of Immunology, Royal Free Hospital School of Medicine, London, UK. Double and triple staining methods for studying the prolifer...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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