Product nameAnti-BrdU antibody (HRP)
See all BrdU primary antibodies
DescriptionSheep polyclonal to BrdU (HRP)
SpecificityThe antibody was tested using immunoprecipitation against 5-Methyl Cytosine (5-MeC) and bromo-deoxyuridine (BrdU) or control (no antigen) and assayed by A-405 spectrophotometry. At a concentration of 25 µg/mL, this product demonstrates 8 fold higher reactivity against BrdU than 5-MeC. This product titers out at 50 ng/mL. For best results, use the product at a concentration of 25 to 100 µg/mL. Nearly complete immunoprecipitation was obtained at a concentration of 100 to 500 µg/mL. This antibody is not species specific. The nucleotide sequence is present in all species.
Tested applicationsSuitable for: ELISA, IHC-P, IHC-Fr, IP, WBmore details
Species reactivityReacts with: a wide range of other species
Bromodeoxyuridine coupled to keyhole limpet hemocyanin (KLH)
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: None
Constituents: 0.15M PBS, pH 7.5
Concentration information loading...
PurityProtein G purified
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
- Immunoassay Blocking (BSA Free) (ab171535)
Our Abpromise guarantee covers the use of ab2285 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
RelevanceThe immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
- Bromodeoxyuridine antibody
- BUdr antibody
This product has been referenced in:
- Walls GV et al. Mice deleted for cell division cycle 73 gene develop parathyroid and uterine tumours: model for the hyperparathyroidism-jaw tumour syndrome. Oncogene 36:4025-4036 (2017). Read more (PubMed: 28288139) »
- Sano H et al. Identification of 5-methylcytosine in DNA fragments immobilized on nitrocellulose paper. Proc Natl Acad Sci U S A 77:3581-5 (1980). Read more (PubMed: 6251470) »