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Chemical/ Small Molecule conjugated to BSA.
Our Abpromise guarantee covers the use of ab8955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.
Using immunocytochemistry, a combination of this antibody and Mouse monoclonal to Mitotic Cells (ab8956) can distinguish and quantitate the four major fractions of the cell cycle.
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/100 - 1/200. ABC method.|
|IHC-P||1/100 - 1/200. ABC method.|
Indirect immunofluorescence staining of BrdU-labeled MR65 lung cancer cells with ab8955.
BrdU staining in sponge Halisarca caerulea using ab8955. Metacrylate embedded tissue sections were incubated with 1:200 diluted primary antibody. An avidin-biotin complex kit using biotinylated rabbit anti-mouse antibody and then incubation in avidin-biotin-peroxidase complex was used for detection.
BrdU staining in paraffin embedded rat intestine using ab8955. Primary antibody diluted 1:200. Peroxidase-conjugated goat anti mouse secondary antibody was used for detection.
Flow cytometric BrdU-DNA cell cycle analysis in a lung cancer cell line. Dilution 1:100 of primary antibody ab8955. FITC-conjugated goat anti mouse secondary antibody was used for detection.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"