Anti-BrdU antibody - Proliferation Marker (ab1893)
Key features and details
- Sheep polyclonal to BrdU - Proliferation Marker
- Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISA
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-BrdU antibody - Proliferation Marker
See all BrdU primary antibodies -
Description
Sheep polyclonal to BrdU - Proliferation Marker -
Host species
Sheep -
Tested applications
Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to BrdU. Coupled to HGG (Human Gamma Globulin).
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General notes
Unstained positive control slides from mice treated with BrdU (formalin-fixed, paraffin-embedded intestine sections) are available as BrdU control slides ab129956.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.60
Constituent: 0.4% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1893 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FrFl | (1) |
Use at an assay dependent concentration.
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IHC-P | (8) |
Use a concentration of 10 µg/ml.
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IHC-Fr | (2) |
Use a concentration of 10 µg/ml.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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IHC-FrFl
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 10 µg/ml. |
IHC-Fr
Use a concentration of 10 µg/ml. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis. -
Cellular localization
Nuclear -
Alternative names
- Bromodeoxyuridine antibody
- BUdr antibody
Images
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Increased numbers of BrdU-labeled NPCs (neural progenitor cells ) with radial GFAP-positive processes in the DG GCL of CRAF ko at P10.
(Panel A shown) Immunohistological analysis of BrdU (green) and the astrocytic marker GFAP (red) stained sagittal brain sections of CRAF ct and CRAF ko hippocampus at P10 24h after a single BrdU application. Representative brain sections of CRAF ct (upper panel) and CRAF ko (lower panel) show BrdU-labeled cells (green) colocalizing with GFAP-positive radial processes (red) (merge, white arrowheads). Scale bar = 50μm.
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ab1893 staining BrdU in Ramos (Human Burkitt's lymphoma cell line) cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).
Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (170)
ab1893 has been referenced in 170 publications.
- Lin H et al. Annexin A2 promotes angiogenesis after ischemic stroke via annexin A2 receptor - AKT/ERK pathways. Neurosci Lett 792:136941 (2023). PubMed: 36367512
- Zeng X et al. Integrated Multi-Omics Analysis Identified PTPRG and CHL1 as Key Regulators of Immunophenotypes in Clear Cell Renal Cell Carcinoma(ccRCC). Front Oncol 12:832027 (2022). PubMed: 35433461
- Leroux S et al. Intermittent hypoxia in a mouse model of apnea of prematurity leads to a retardation of cerebellar development and long-term functional deficits. Cell Biosci 12:148 (2022). PubMed: 36068642
- Hao K et al. Nicotinamide reverses deficits in puberty-born neurons and cognitive function after maternal separation. J Neuroinflammation 19:232 (2022). PubMed: 36131290
- Li WY et al. Krüppel-like factor 7 attenuates hippocampal neuronal injury after traumatic brain injury. Neural Regen Res 17:661-672 (2022). PubMed: 34380908