Anti-BrdU antibody - Proliferation Marker (ab1893)

Overview

  • Product name
    Anti-BrdU antibody - Proliferation Marker
    See all BrdU primary antibodies
  • Description
    Sheep polyclonal to BrdU - Proliferation Marker
  • Host species
    Sheep
  • Tested applications
    Suitable for: IHC-FrFl, IHC-P, IHC-Fr, ICC/IF, ELISA, WB, IHC-FoFrmore details
  • Immunogen

    Other Immunogen Type corresponding to BrdU. Bromodeoxyuridine coupled to HGG (Human Gamma Globulin)

Properties

Applications

Our Abpromise guarantee covers the use of ab1893 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
IHC-P Use a concentration of 10 µg/ml.
IHC-Fr Use a concentration of 10 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 21118958
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19332057

Target

  • Relevance
    The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis.
  • Cellular localization
    Nuclear
  • Alternative names
    • Bromodeoxyuridine antibody
    • BUdr antibody

Images

  • ab1893 staining BrdU in COS7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 2% BSA for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in 2% BSA) for 1 hour at 25°C. ab96945, a DyLight® 594-conjugated rabbit anti-sheep IgG (H+L) polyclonal was used as the secondary antibody (1/200). Nuclei are stained blue with DAPI.

    See Abreview

  • ab1893 staining BrdU in Ramos cell line xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 15% serum for 1 hour at 20°C; antigen retrieval was by heat mediation in a sodium citrate buffer, pH 6. Samples were incubated with primary antibody (1/260 in TBS) for 18 hours at 20°C. An undiluted Alexa Fluor® 488-conjugated donkey anti-sheep IgG polyclonal was used as the secondary antibody.

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References

This product has been referenced in:
  • Pfeiffer V  et al. Impaired neuronal maturation of hippocampal neural progenitor cells in mice lacking CRAF. PLoS One 13:e0192067 (2018). Read more (PubMed: 29590115) »
  • Majewski L  et al. Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. Nucleus 9:125-141 (2018). Read more (PubMed: 29293066) »

See all 83 Publications for this product

Customer reviews and Q&As

Unfortunately we do not have any cross-reactivity data against FDU.

Use 0.1M Borate buffer. Recipe is:

Sodium Tetraborate (fw =381.4) --> 38.14 grams to 1000 ml water, pH 8.5

After the soaking in the Borate buffer (pH 8.5) they are neutralized by rinsing in the PBS buffer (pH 7.4), which is listed...

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Cultured cells should be pulsed with 10uM BrdU for 1 hour - overnight.

The Brdu antibody should recognize both incorporated and free Brdu. The free Brdu should wash away in the experiment.

This BrdU antibody is not species specific.

It appears that standard BrdU incorporation (similar to thymidine incorporation) should be sufficient. There are many published protocols for this type of assay. As far a denaturing goes, DNA can be denatured after the gel is run. Procedures that expos...

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Standard WB protocol should be sufficient for this antibody. We prefer the ECL based format. The DNA needs to be SS for the antibody to recognize the target, so you will need to be sure the DNA is denatured either with acid/base steps. I would also try...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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