Product nameBrdU Immunohistochemistry Kit
Sample typeTissue, Adherent cells, Suspension cells
ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.
The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.
Control Slides: Mouse intestinal tissue pulsed with BrdU ab129956
*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution)) is not provided in this kit.
Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 kit 4X Trypsin Enzyme Concentrate 1 x 3ml Blocking Buffer 1 x 6ml Control Slides (stained) 1 slide Control Slides (unstained) 4 slides DAB Solution 1 (DAB Concentrate) 1 x 0.3ml DAB Solution 2 (Substrate Reaction Buffer) 1 x 6ml Denaturing Solution 1 x 6ml Detector Antibody (Biotinylated and pre-diluted) 1 x 6ml Hematoxylin Counterstain 1 x 6ml Mounting Media 1 x 6ml Streptavidin-HRP Conjugate (pre-diluted) 1 x 6ml Trypsin Dilution Buffer 1 x 12ml
Our Abpromise guarantee covers the use of ab125306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
This product has been referenced in:
- Norum JH et al. The tankyrase inhibitor G007-LK inhibits small intestine LGR5+stem cell proliferation without altering tissue morphology. Biol Res 51:3 (2018). Mouse . Read more (PubMed: 29316982) »
- Gou Y et al. Protein Arginine Methyltransferase PRMT1 Is Essential for Palatogenesis. J Dent Res N/A:22034518785164 (2018). Read more (PubMed: 29986157) »