Overview

  • Product name
    BrdU Immunohistochemistry Kit
  • Sample type
    Tissue, Adherent cells, Suspension cells
  • Product overview

    ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.


    The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.


    Control Slides: Mouse intestinal tissue pulsed with BrdU ab129956 


     

  • Notes

    *Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution)) is not provided in this kit.

     

     

  • Tested applications
    Suitable for: IHC-Fr, IHC-Pmore details

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab125306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

Images

  • Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.
  • Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.
  • Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.

Protocols

References

This product has been referenced in:
  • Norum JH  et al. The tankyrase inhibitor G007-LK inhibits small intestine LGR5+stem cell proliferation without altering tissue morphology. Biol Res 51:3 (2018). Mouse . Read more (PubMed: 29316982) »
  • Gou Y  et al. Protein Arginine Methyltransferase PRMT1 Is Essential for Palatogenesis. J Dent Res N/A:22034518785164 (2018). Read more (PubMed: 29986157) »
See all 9 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Abreviews
clean-grade SD rat was fasted from 12 h prior to the establishment of the pancreatitis model until 24 h after the model was established, but drinking water was made continuously available. The rat resumed regular intake of food and water thereafter. On day 7 after pancreatitis was induced, the rat was sacrificed by cervical dislocation. At 6 h before the rat was sacrificed and tissue samples were collected, BrdU was administered via IP injection at 100 mg/kg body weight.

After 7 days of the establishment of pancreatitis, BrdU-positive cells formed cell sheets.

Abcam user community

Verified customer

Submitted Aug 07 2015

BrdU immunohistochemistry kit

Poor Excellent 5/5 (Ease of Use)
Abreviews
Very easy to use, however disappointing results (high non-specific background staining) when used exactly as per kit instructions. Poor compared to ab2284 (also shown in image) which was tested in parallel and which worked very nicely.

Abcam user community

Verified customer

Submitted Aug 27 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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