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    brdu-immunohistochemistry-kit-ab125306.pdf

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BrdU Immunohistochemistry Kit (ab125306)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (2)Q&A (4)References (18)

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BrdU Immunohistochemistry Kit (ab125306)
  • BrdU Immunohistochemistry Kit (ab125306)

Key features and details

  • Sample type: Adherent cells, Suspension cells, Tissue

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Overview

  • Product name

    BrdU Immunohistochemistry Kit
  • Sample type

    Tissue, Adherent cells, Suspension cells
  • Product overview

    ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.


    The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.


    Control Slides: BrdU labeled MCF7 (human breast carcinoma derived) cells grown on glass slides (ab281335).


     

  • Notes

    *Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution) is not provided in this kit.

     

     

  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 kit
    4X Trypsin Enzyme Concentrate 1 x 3ml
    Blocking Buffer 1 x 6ml
    Control Slides (stained) 1 slide
    Control Slides (unstained) 4 slides
    DAB Solution 1 (DAB Concentrate) 1 x 0.3ml
    DAB Solution 2 (Substrate Reaction Buffer) 1 x 6ml
    Denaturing Solution 1 x 6ml
    Detector Antibody (Biotinylated and pre-diluted) 1 x 6ml
    Hematoxylin Counterstain 1 x 6ml
    Mounting Media 1 x 6ml
    Streptavidin-HRP Conjugate (pre-diluted) 1 x 6ml
    Trypsin Dilution Buffer 1 x 12ml
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Markers
    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA / Nucleotides
    • Kits/ Lysates/ Other
    • Tools and Reagents
    • IHC Tools/ Reagents
    • Kits/ Lysates/ Other
    • Kits
    • IHC Kits
    • Kits/ Lysates/ Other
    • Kits
    • IHC tools
    • Counterstains and Special stains
    • BrdU

Associated products

  • Related Products

    • Anti-BrdU antibody [BU1/75 (ICR1)] - Proliferation Marker (ab6326)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab125306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

Images

  • BrdU Immunohistochemistry Kit (ab125306)
    BrdU Immunohistochemistry Kit (ab125306)

    Formalin-fixed, paraffin embedded  MCF7 cells sections stained using ab125306. More diffuse nuclear immunostaining can be seen here.

  • BrdU Immunohistochemistry Kit (ab125306)
    BrdU Immunohistochemistry Kit (ab125306)

    Formalin-fixed, paraffin embedded  MCF7 cells sections stained using ab125306. A more localized nuclear staining reaction can be seen here.

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (18)

Publishing research using ab125306? Please let us know so that we can cite the reference in this datasheet.

ab125306 has been referenced in 18 publications.

  • Suzuki K  et al. Deletion of B-cell translocation gene 2 (BTG2) alters the responses of glial cells in white matter to chronic cerebral hypoperfusion. J Neuroinflammation 18:86 (2021). PubMed: 33812385
  • Park NR  et al. Reticulocalbin 3 is involved in postnatal tendon development by regulating collagen fibrillogenesis and cellular maturation. Sci Rep 11:10868 (2021). PubMed: 34035379
  • Chuang SC  et al. G Protein-Coupled Estrogen Receptor Mediates Cell Proliferation through the cAMP/PKA/CREB Pathway in Murine Bone Marrow Mesenchymal Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32899453
  • Tan CL  et al. Nicotinamide Metabolism Modulates the Proliferation/Differentiation Balance and Senescence of Human Primary Keratinocytes. J Invest Dermatol 139:1638-1647.e3 (2019). PubMed: 30776433
  • Matsui S  et al. Interleukin-13 and its signaling pathway is associated with obesity-related colorectal tumorigenesis. Cancer Sci 110:2156-2165 (2019). PubMed: 31099450
View all Publications for this product

Customer reviews and Q&As

Show All Reviews Q&A
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1-2 of 2 Abreviews

Review for BrdU Immunohistochemistry Kit (ab125306)

Good Good 4/5 (Ease of Use)
Abreviews
Abreviews
abreview image
clean-grade SD rat was fasted from 12 h prior to the establishment of the pancreatitis model until 24 h after the model was established, but drinking water was made continuously available. The rat resumed regular intake of food and water thereafter. On day 7 after pancreatitis was induced, the rat was sacrificed by cervical dislocation. At 6 h before the rat was sacrificed and tissue samples were collected, BrdU was administered via IP injection at 100 mg/kg body weight.

After 7 days of the establishment of pancreatitis, BrdU-positive cells formed cell sheets.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 07 2015

BrdU immunohistochemistry kit

Poor Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Very easy to use, however disappointing results (high non-specific background staining) when used exactly as per kit instructions. Poor compared to ab2284 (also shown in image) which was tested in parallel and which worked very nicely.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 27 2014

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