BrdU Immunohistochemistry Kit (ab125306)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
BrdU Immunohistochemistry Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Product overview
ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.
The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.
Control Slides: Mouse intestinal tissue pulsed with BrdU ab281335
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Notes
*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution)) is not provided in this kit.
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Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit 4X Trypsin Enzyme Concentrate 1 x 3ml Blocking Buffer 1 x 6ml Control Slides (stained) 1 slide Control Slides (unstained) 4 slides DAB Solution 1 (DAB Concentrate) 1 x 0.3ml DAB Solution 2 (Substrate Reaction Buffer) 1 x 6ml Denaturing Solution 1 x 6ml Detector Antibody (Biotinylated and pre-diluted) 1 x 6ml Hematoxylin Counterstain 1 x 6ml Mounting Media 1 x 6ml Streptavidin-HRP Conjugate (pre-diluted) 1 x 6ml Trypsin Dilution Buffer 1 x 12ml -
Research areas
Associated products
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
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| IHC-P |
Use at an assay dependent concentration.
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| Notes |
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IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
Images
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BrdU Immunohistochemistry Kit (ab125306)
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BrdU Immunohistochemistry Kit (ab125306)Formalin-fixed, paraffin embedded MCF7 cells sections stained using ab125306. A more localized nuclear staining reaction can be seen here.
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab125306 has been referenced in 13 publications.
- Matsui S et al. Interleukin-13 and its signaling pathway is associated with obesity-related colorectal tumorigenesis. Cancer Sci 110:2156-2165 (2019). PubMed: 31099450
- Zhang J et al. Role of surface ectoderm-specific mitofusin 2 in the corneal morphologic development of mice. Am J Transl Res 11:3620-3628 (2019). PubMed: 31312372
- Yang SH et al. CD24 expression indicates healthier phenotype and less tendency of cellular senescence in human nucleus pulposus cells. Artif Cells Nanomed Biotechnol 47:3021-3028 (2019). PubMed: 31334674
- Hu L et al. PLF-1 (Proliferin-1) Modulates Smooth Muscle Cell Proliferation and Development of Experimental Intimal Hyperplasia. J Am Heart Assoc 8:e005886 (2019). PubMed: 31838975
- Norum JH et al. The tankyrase inhibitor G007-LK inhibits small intestine LGR5+stem cell proliferation without altering tissue morphology. Biol Res 51:3 (2018). Mouse . PubMed: 29316982