BrdU Immunohistochemistry Kit (ab125306)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameBrdU Immunohistochemistry Kit
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Sample typeTissue, Adherent cells, Suspension cells
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Product overview
ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.
The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.
Control Slides: Mouse intestinal tissue pulsed with BrdU ab129956
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Notes
*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution)) is not provided in this kit.
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Tested applicationsSuitable for: IHC-Fr, IHC-Pmore details
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 kit 4X Trypsin Enzyme Concentrate 1 x 3ml Blocking Buffer 1 x 6ml Control Slides (stained) 1 slide Control Slides (unstained) 4 slides DAB Solution 1 (DAB Concentrate) 1 x 0.3ml DAB Solution 2 (Substrate Reaction Buffer) 1 x 6ml Denaturing Solution 1 x 6ml Detector Antibody (Biotinylated and pre-diluted) 1 x 6ml Hematoxylin Counterstain 1 x 6ml Mounting Media 1 x 6ml Streptavidin-HRP Conjugate (pre-diluted) 1 x 6ml Trypsin Dilution Buffer 1 x 12ml -
Research areas
Associated products
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Related Products
Applications
Our Abpromise guarantee covers the use of ab125306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. |
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - BrdU Immunohistochemistry Kit (ab125306)Formalin-fixed, paraffin embedded mouse intestinal tissue sections stained using ab125306.
Protocols
References
This product has been referenced in:
- Norum JH et al. The tankyrase inhibitor G007-LK inhibits small intestine LGR5+stem cell proliferation without altering tissue morphology. Biol Res 51:3 (2018). Mouse . Read more (PubMed: 29316982) »
- Gou Y et al. Protein Arginine Methyltransferase PRMT1 Is Essential for Palatogenesis. J Dent Res N/A:22034518785164 (2018). Read more (PubMed: 29986157) »
