BrdU Immunohistochemistry Kit (ab125306)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
-
Product name
BrdU Immunohistochemistry Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Product overview
ab125306 is a histochemical staining kit for the detection and localization of bromodeoxyuridine incorporated into newly synthesized DNA of actively proliferating cells.
The material in ab125306 is sufficient to run 50 slides. The average test area is defined as a circle around the tissue with an approximate diameter of 2 centimeters.
Control Slides: BrdU labeled MCF7 (human breast carcinoma derived) cells grown on glass slides (ab281335).
-
Notes
*Trypsin is only required if using formalin fixed tissues. If the tissues are fixed in alcohol, trypsin digestion is not required. Please note that a quenching solution (hydrogen peroxide (30% solution) is not provided in this kit.
-
Tested applications
Suitable for: IHC-Fr, IHC-Pmore details
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 kit 4X Trypsin Enzyme Concentrate 1 x 3ml Blocking Buffer 1 x 6ml Control Slides (stained) 1 slide Control Slides (unstained) 4 slides DAB Solution 1 (DAB Concentrate) 1 x 0.3ml DAB Solution 2 (Substrate Reaction Buffer) 1 x 6ml Denaturing Solution 1 x 6ml Detector Antibody (Biotinylated and pre-diluted) 1 x 6ml Hematoxylin Counterstain 1 x 6ml Mounting Media 1 x 6ml Streptavidin-HRP Conjugate (pre-diluted) 1 x 6ml Trypsin Dilution Buffer 1 x 12ml -
Research areas
Associated products
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IHC-P |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
|
IHC-P
Use at an assay dependent concentration. |
Images
-
BrdU Immunohistochemistry Kit (ab125306)
Formalin-fixed, paraffin embedded MCF7 cells sections stained using ab125306. More diffuse nuclear immunostaining can be seen here.
-
BrdU Immunohistochemistry Kit (ab125306)Formalin-fixed, paraffin embedded MCF7 cells sections stained using ab125306. A more localized nuclear staining reaction can be seen here.
Datasheets and documents
-
SDS download
-
Datasheet download
References (18)
ab125306 has been referenced in 18 publications.
- Suzuki K et al. Deletion of B-cell translocation gene 2 (BTG2) alters the responses of glial cells in white matter to chronic cerebral hypoperfusion. J Neuroinflammation 18:86 (2021). PubMed: 33812385
- Park NR et al. Reticulocalbin 3 is involved in postnatal tendon development by regulating collagen fibrillogenesis and cellular maturation. Sci Rep 11:10868 (2021). PubMed: 34035379
- Chuang SC et al. G Protein-Coupled Estrogen Receptor Mediates Cell Proliferation through the cAMP/PKA/CREB Pathway in Murine Bone Marrow Mesenchymal Stem Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32899453
- Tan CL et al. Nicotinamide Metabolism Modulates the Proliferation/Differentiation Balance and Senescence of Human Primary Keratinocytes. J Invest Dermatol 139:1638-1647.e3 (2019). PubMed: 30776433
- Matsui S et al. Interleukin-13 and its signaling pathway is associated with obesity-related colorectal tumorigenesis. Cancer Sci 110:2156-2165 (2019). PubMed: 31099450