Question (75217) | BrdU Immunohistochemistry Kit (ab125306)

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Question

Inquiry: For ab125306-BrdU Immunohistochemistry Kit, I have frozen tissue section. Would you mind telling me what procedure for frozen section at the beginning.

Answer

Following the staining protocol on page 11 of the protocol for ab125306, with frozen tissue section you would skip step 1 "Deparaffinization" and start with step 2.1. You will also skip step 2.2, as you do not need to perform antigen retrieval unless your tissues were formalin fixed. If you have PFA perfusion fixed, frozen tissue you will most likely still need to included step 2.2, but if you used a fixative such as methanol, ethanol, or acetone you can skip step 2.2.

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