Review for BrdU Immunohistochemistry Kit (ab125306)

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clean-grade SD rat was fasted from 12 h prior to the establishment of the pancreatitis model until 24 h after the model was established, but drinking water was made continuously available. The rat resumed regular intake of food and water thereafter. On day 7 after pancreatitis was induced, the rat was sacrificed by cervical dislocation. At 6 h before the rat was sacrificed and tissue samples were collected, BrdU was administered via IP injection at 100 mg/kg body weight.

After 7 days of the establishment of pancreatitis, BrdU-positive cells formed cell sheets.

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Submitted Aug 07 2015

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