Brefeldin A, Inhibitor of ADP-ribosylation factor (ab120299)

Overview

  • Product name
    Brefeldin A, Inhibitor of ADP-ribosylation factor
  • Description
    Inhibitor of ADP-ribosylation factor
  • Alternative names
    • Ascotoxin
    • BFA
    • Cyanein
    • Decumbin
  • Biological description
    Reversible inhibitor of protein translocation from the endoplasmic reticulum to the Golgi apparatus. Inhibits binding of the cytosolic coat protein, β-COP and ADP-ribosylation factor (ARF) to Golgi membranes and inhibits GDP-GTP exchange.
  • Purity
    > 98%

Properties

Applications

Our Abpromise guarantee covers the use of ab120299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab84340 staining golgin-97 in MCF7 cells treated with brefeldin A (ab120299), by ICC/IF. Increase in Golgin-97 expression correlates with increased concentration of brefeldin A, as described in literature.
    The cells were incubated at 37°C for 1.5 h in media containing different concentrations of ab120299 (brefeldin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab84340 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Lippincott-Schwartz J  et al. Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell 67:601-16 (1991). Read more (PubMed: 1682055) »
  • Donaldson JG  et al. Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus. J Cell Biol 112:579-88 (1991). Read more (PubMed: 1993732) »
  • Pelham HR Multiple targets for brefeldin A. Cell 67:449-51 (1991). Read more (PubMed: 1934055) »
See 1 Publication for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Sub-cellular frationation followed by WB of the protein of interest confirmed reduction of ER to Golgi translocation of the protein of interest. Incubated with 10ug/ml of Brefeldin A for 4 hours.

Abcam user community

Verified customer

Submitted May 29 2017

Answer

We would always recommend storing the reconstituted product at -20ºC until it is ready for use as other suppliers also suggest. Generally, these aliquots will be useable for up to one month. Some customers still use the product for up to 3 months but longer may increase the loss of potency.

Once thawed we would recommend using it immediately and have not tested any other storage conditions.

Having done a literature search, it seems like some researchers store it at 4ºC whilst they do their different experiments. Please note however that DMSO freezes at this temperature and so the solution needs to be completely thawed each time. This is why we would not recommend doing this often as every time you undergo a freeze thaw cycle you decrease the quality of the product so this should be kept to a minimum and experiments planned accordingly to try and decrease the number of freeze thaw cycles with one aliquot.

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Answer

Thank you for contacting us.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase. For the mentioned TNF alpha antibodies I can confirm that they can be used for intracytoplasmic staining in flow cytometry.

Please note that ab11458 requires membrane permeabilisation, as mentioned on the datasheet. And in order to prevent secretion of the cytokine(s), an inhibitor such as Brefeldin A (ab120299) is recommended.

ab120299: Click here (or use the following: https://www.abcam.com/index.html?datasheet=120299).

We like to encourage you to submit an Abreview about one of the antibodies via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates. To find out more about our Abreview system, please see the following link: https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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