Brefeldin A, Inhibitor of ADP-ribosylation factor (ab120299)

Overview

  • Product name
    Brefeldin A, Inhibitor of ADP-ribosylation factor
  • Description
    Inhibitor of ADP-ribosylation factor
  • Alternative names
    • Ascotoxin
    • BFA
    • Cyanein
    • Decumbin
  • Biological description
    Reversible inhibitor of protein translocation from the endoplasmic reticulum to the Golgi apparatus. Inhibits binding of the cytosolic coat protein, β-COP and ADP-ribosylation factor (ARF) to Golgi membranes and inhibits GDP-GTP exchange.
  • Purity
    > 98%
  • CAS Number
    20350-15-6
  • Chemical structure
    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab84340 staining golgin-97 in MCF7 cells treated with brefeldin A (ab120299), by ICC/IF. Increase in Golgin-97 expression correlates with increased concentration of brefeldin A, as described in literature.
    The cells were incubated at 37°C for 1.5 h in media containing different concentrations of ab120299 (brefeldin A) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab84340 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:
  • Lippincott-Schwartz J  et al. Brefeldin A's effects on endosomes, lysosomes, and the TGN suggest a general mechanism for regulating organelle structure and membrane traffic. Cell 67:601-16 (1991). Read more (PubMed: 1682055) »
  • Donaldson JG  et al. Guanine nucleotides modulate the effects of brefeldin A in semipermeable cells: regulation of the association of a 110-kD peripheral membrane protein with the Golgi apparatus. J Cell Biol 112:579-88 (1991). Read more (PubMed: 1993732) »
  • Pelham HR Multiple targets for brefeldin A. Cell 67:449-51 (1991). Read more (PubMed: 1934055) »
See 1 Publication for this product

Customer reviews and Q&As

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Sub-cellular frationation followed by WB of the protein of interest confirmed reduction of ER to Golgi translocation of the protein of interest. Incubated with 10ug/ml of Brefeldin A for 4 hours.

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Verified customer

Submitted May 29 2017

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