Brefeldin A Solution (1,000X) (ab193369)
Overview
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Product name
Brefeldin A Solution (1,000X) -
Product overview
Formulation: Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration: 5.0 mg/ml
Storage: Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Recommended Usage: Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.
Application: Intracellular staining for flow cytometry
Notes: Completely thaw the solution before use, DMSO freezes at 4°C. We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.
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Notes
Brefeldin A is a protein transport inhibitor that increases intracellular cytokine staining signals by blocking transport processes during activation of a cell. This leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum, and makes Brefeldin A useful in intracellular staining of cytokines.
Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest. Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium. -
Tested applications
Suitable for: Flow Cytmore details
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 ml Brefeldin A Solution (1,000X) 1 x 1ml
Associated products
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Related Buffer
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab193369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
1/1000 Dilute the 1000X solution ti 1X in the tissue culture medium/ It is recomended that cells are cultured with Brefeldin A for 24 hours. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. 1/1000 Dilute the 1000X solution ti 1X in the tissue culture medium/ It is recomended that cells are cultured with Brefeldin A for 24 hours. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab193369 has been referenced in 3 publications.
- Vinhas A et al. Magnetic Stimulation Drives Macrophage Polarization in Cell to-Cell Communication with IL-1ß Primed Tendon Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32751697
- Chansard A et al. Unveiling Interindividual Variability of Human Fibroblast Innate Immune Response Using Robust Cell-Based Protocols. Front Immunol 11:569331 (2020). PubMed: 33505391
- Akhter N et al. Oxidative Stress Induces Expression of the Toll-Like Receptors (TLRs) 2 and 4 in the Human Peripheral Blood Mononuclear Cells: Implications for Metabolic Inflammation. Cell Physiol Biochem 53:1-18 (2019). PubMed: 31162913