Product nameBrefeldin A Solution (1,000X)
Formulation: Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium.
Concentration: 5.0 mg/ml
Storage: Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature.
Recommended Usage: Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability.
Application: Intracellular staining for flow cytometry
Notes: Completely thaw the solution before use, DMSO freezes at 4°C. We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.
Brefeldin A is a protein transport inhibitor that increases intracellular cytokine staining signals by blocking transport processes during activation of a cell. This leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum, and makes Brefeldin A useful in intracellular staining of cytokines.
Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest. Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.
Tested applicationsSuitable for: Flow Cytmore details
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 ml Brefeldin A Solution (1,000X) 1 x 1ml
Our Abpromise guarantee covers the use of ab193369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
1/1000 Dilute the 1000X solution ti 1X in the tissue culture medium/ It is recomended that cells are cultured with Brefeldin A for 24 hours.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab193369 has not yet been referenced specifically in any publications.