Overview

  • Product name
    Brefeldin A Solution (1,000X)
  • Product overview

    Formulation: Brefeldin A Solution is supplied as a 1000X in DMSO, which should be diluted to 1X in cell culture medium. 

    Concentration: 5.0 mg/ml 

    Storage: Store the Brefeldin A Solution (1,000X) between 2°C and 8°C. Note: DMSO freezes at this temperature. 

    Recommended Usage: Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with brefeldin A for ≤ 24 hours, as this can become toxic for cell viability. 

    Application: Intracellular staining for flow cytometry 

    Notes: Completely thaw the solution before use, DMSO freezes at 4°C. We recommend aliquotting the reagent into smaller volumes to avoid repeated freeze-thawing.

     

  • Notes

    Brefeldin A is a protein transport inhibitor that increases intracellular cytokine staining signals by blocking transport processes during activation of a cell. This leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum, and makes Brefeldin A useful in intracellular staining of cytokines.
    Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest. Brefeldin A Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

     

  • Tested applications
    Suitable for: Flow Cytmore details

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 ml
    Brefeldin A Solution (1,000X) 1 x 1ml

Applications

Our Abpromise guarantee covers the use of ab193369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

1/1000 Dilute the 1000X solution ti 1X in the tissue culture medium/ It is recomended that cells are cultured with Brefeldin A for 24 hours.

References

ab193369 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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