Overview

  • Product name

  • Description

    Rabbit polyclonal to Brevican
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 83-262 of Human Brevican (AAH09117).

  • Positive control

    • Human breast carcinoma tissue; MCF7 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab111719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 99 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Brevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C-terminal epitope EAMESE.
  • Cellular localization

    Isoform 1 is secreted, extracellular space, extracellular matrix; Isoform 2 is found on the membrane; Lipid-anchor, GPI-anchor.
  • Database links

  • Alternative names

    • BCAN antibody
    • BEHAB antibody
    • Brain enriched hyaluronan binding protein antibody
    • Brevican core protein antibody
    • Brevican core protein isoform 1 antibody
    • Brevican core protein isoform 2 antibody
    • Brevican proteoglycan antibody
    • Chondroitin sulfate proteoglycan 7 antibody
    • Chondroitin sulfate proteoglycan BEHAB antibody
    • CSPG7 antibody
    • MGC13038 antibody
    see all

Images

  • Anti-Brevican antibody (ab111719) at 1/500 dilution + MCF-7 whole cell lysate
    at 30 µg

    Predicted band size: 99 kDa



    7.5% SDS-PAGE.
  • ab111719 at 1/500 dilution staining Brevican in Formalin-fixed, paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Felemban M  et al. Extracellular matrix component expression in human pluripotent stem cell-derived retinal organoids recapitulates retinogenesis in vivo and reveals an important role for IMPG1 and CD44 in the development of photoreceptors and interphotoreceptor matrix. Acta Biomater 74:207-221 (2018). Read more (PubMed: 29777959) »
  • Orlando C  et al. Perisynaptic chondroitin sulfate proteoglycans restrict structural plasticity in an integrin-dependent manner. J Neurosci 32:18009-17, 18017a (2012). ICC/IF ; Mouse . Read more (PubMed: 23238717) »
See all 2 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for submitting an Abreview for ab111719. As you may have noticed, your review has now been published on our website.

Since you obtained poor results using the antibody in an untested application, we would like to follow up on this to see if we can possibly improve the results you are seeing with this antibody.

Based on the information in your review, I feel that altering the following step in your protocol may help improve your results:
- try another fixation method (e.g. methanol or acetone)
- do a no primary control to check how much background comes from the secondary antibody alone
- I can't tell for sure if a confocal microscope was used for your images. If not, it might be worthwhile trying, since you would be able to move the focal plane through the cell and could be able to see the extracellular staining better and distinguish between cytoplasmic background and membrane/ECM staining.

If you decide to follow these suggestions, please let me know if they are helpful.
I wish you good luck with your research.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cultured Cells (human astrocytes)
Specification
human astrocytes
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% triton
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 05 2012

Question
Answer

Thank you for contacting us.

Do you happen to know which region of Brevican is membrane bound?The immunogen for ab111719 falls within the region betweenamino acids 83 and 262 of human Brevican. According to the SwissProt entry for this protein (Q96GW7), this region encompasses a portion of the Ig-like V-type domain, the entire link 1 domain and a portion of the link 2 domain. However, I am unsure if any of these domains are involved in membrane binding.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Question
Answer

DISCOUNT CODE: *****
Expiration date: **********

I am very pleased to hear you would like to accept our offer and test ab111719 in ICC/IF. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview forICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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