Key features and details
- Mouse polyclonal to Brevican
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Brevican antibody
See all Brevican primary antibodies
DescriptionMouse polyclonal to Brevican
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Full length Brevican protein (Human)
- Brevican transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67580 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceBrevican is the most abundant chondroitin sulfate proteoglycan in the extracellular matrix of the adult brain. It is a member of the lectican family of aggregating extracellular matrix proteoglycans that bear chondroitin sulfate (CS) chains. It is highly expressed in the central nervous system and is thought to stabilize synapses and inhibit neural plasticity. Brevican is secreted from astrocytes and neurons as a 145 kD core protein that bears up to three, covalently-linked, CS chains. It is also is secreted as a 145 kD core protein without CS chains. When cleaved by extracellular glutamyl endopeptidases, the ADAMTSs, a 55 kD N-terminal fragment is formed that contains the unique C-terminal epitope EAMESE.
Cellular localizationIsoform 1 is secreted, extracellular space, extracellular matrix; Isoform 2 is found on the membrane; Lipid-anchor, GPI-anchor.
- BCAN antibody
- BEHAB antibody
- Brain enriched hyaluronan binding protein antibody
All lanes : Anti-Brevican antibody (ab67580) at 1/500 dilution
Lane 1 : Brevican transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 99 kDa
Observed band size: 170 kDa why is the actual band size different from the predicted?
Additional bands at: 125 kDa. We are unsure as to the identity of these extra bands.
ab67580 has been referenced in 1 publication.
- Lima MA et al. Prognostic Value of ADAMTS Proteases and Their Substrates in Epithelial Ovarian Cancer. Pathobiology 83:316-26 (2016). PubMed: 27359117