Product nameAnti-BRF1 antibody
See all BRF1 primary antibodies
DescriptionRabbit polyclonal to BRF1
SpecificityThis antibody recognizes BRF1
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Rat, Cow, Dog, Saccharomyces cerevisiae, Drosophila melanogaster
- NIH3T3 lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab49122 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay was 1:62500.
|WB||Use a concentration of 1.25 µg/ml. Predicted molecular weight: 74 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
FunctionGeneral activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
Sequence similaritiesBelongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
- Information by UniProt
- B related factor 1 antibody
- B-related factor 1 antibody
- BRF antibody
Anti-BRF1 antibody (ab49122) at 1.25 µg/ml + NIH3T3 lysate at 10 µg with 5% skimmed milk in PBS.
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 74 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
Gel concentration 12%
ab49122 has not yet been referenced specifically in any publications.