- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-BRF1 antibody
See all BRF1 primary antibodies -
DescriptionRabbit polyclonal to BRF1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide, mapping to a region between amino acids 550-600 of Human BRF1 (NP_001510.2)
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Positive control
- HeLa or 293T whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab74221 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 74 kDa. | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionGeneral activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.
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Sequence similaritiesBelongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 2972 Human
- Omim: 604902 Human
- SwissProt: Q92994 Human
- Unigene: 424484 Human
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Alternative names
- B related factor 1 antibody
- B-related factor 1 antibody
- BRF antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRF1 antibody (ab74221)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling BRF1 with ab74221 at 1/200 (1µg/ml). Detection: DAB. -
Western blot - Anti-BRF1 antibody (ab74221)All lanes : Anti-BRF1 antibody (ab74221) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 74 kDa
Observed band size: 74 kDa
Additional bands at: 140 kDa, 200 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation - Anti-BRF1 antibody (ab74221)HeLa whole cell lysate (1mg) was subjected to immunoprecipitation using ab74221 (3µg/mg lysate; lane 1) or a control IgG antibody (lane 2). 20% of the immunoprecipitates were used for Western blotting and bands were identified using ab74221 at a concentration of 1µg/ml. A band at the predicted molecular weight of 74kDa was detected using chemiluminescence with an exposure time of 3 seconds.
References
This product has been referenced in:
- Lee YL et al. MAF1 represses CDKN1A through a Pol III-dependent mechanism. Elife 4:e06283 (2015). ChIP ; Human . Read more (PubMed: 26067234) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"