Product nameAnti-BRF2 antibody
See all BRF2 primary antibodies
DescriptionRabbit polyclonal to BRF2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment, corresponding to a region within amino acids 2-235 of Human BRF2 (Uniprot ID: Q9HAW0).
- A549, HeLa, HepG2 and HCT116 whole cell lysates; HeLa cells; Human Ca922 xenograft tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 47 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Or perform heat mediated antigen retrieval with Tris-EDTA buffer pH 8.|
|ICC/IF||1/100 - 1/1000.|
FunctionGeneral activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
Sequence similaritiesBelongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
- Information by UniProt
- B-related factor 2 antibody
- BRF-2 antibody
- BRF2 antibody
All lanes : Anti-BRF2 antibody (ab154658) at 1/1000 dilution
Lane 1 : A549 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : HCT116 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 47 kDa
10% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labeling BRF2 with ab154658 at 1/200 dilution. Lower image is co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human Ca922 xenograft tissue labeling BRF2 with ab154658 at 1/500 dilution.
ab154658 has not yet been referenced specifically in any publications.