Overview

  • Product name

  • Description

    Goat polyclonal to BRF2
  • Host species

    Goat
  • Tested applications

    Suitable for: ChIP, IP, ELISA, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide: QAARQAATSVPNPP, corresponding to C terminal amino acids 406-419 of Human BRF2.

  • Positive control

    • HeLa cell lysate. Human placenta lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab17011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 20418882
ELISA 1/16000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).

Target

  • Function

    General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
  • Sequence similarities

    Belongs to the TFIIB family.
    Contains 1 TFIIB-type zinc finger.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • B-related factor 2 antibody
    • BRF-2 antibody
    • BRF2 antibody
    • BRF2_HUMAN antibody
    • BRFU antibody
    • FLJ11052 antibody
    • hBRFU antibody
    • hTFIIIB50 antibody
    • RNA polymerase III transcription initiation factor BRF2 antibody
    • TFIIIB50 antibody
    • Transcription factor IIB- related factor TFIIIB50 antibody
    • Transcription factor IIIB 50 kDa subunit antibody
    see all

Images

  • ab17011 at 1.5µg/ml detecting BRF2 from HeLa cell lysate (35ug total protein per lane) by Western blot. Primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.

     

    ab17011 staining (1.5µg/ml) of Hela lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • ab17011 used in ChIP. For details please see Abreview.

References

This product has been referenced in:

  • Gouge J  et al. Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. Cell 163:1375-87 (2015). Read more (PubMed: 26638071) »
  • Lockwood WW  et al. Integrative genomic analyses identify BRF2 as a novel lineage-specific oncogene in lung squamous cell carcinoma. PLoS Med 7:e1000315 (2010). WB ; Human . Read more (PubMed: 20668658) »
See all 3 Publications for this product

Customer reviews and Q&As

Application
ChIP
Sample
Human Cell lysate - nuclear (HeLa S3)
Specification
HeLa S3
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 30 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde, 1%
Detection step
Real-time PCR
Positive control
Human U6 locus, chr15:65919436-65919686 (hg18)
Negative control
Gene desert locus, chr10:43939489-43939659 (hg18)

Dr. Andrew Oler

Verified customer

Submitted Apr 13 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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