Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab17011 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration. PubMed: 20418882|
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).Can be blocked with Human BRF2 peptide (ab23297).|
FunctionGeneral activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
Sequence similaritiesBelongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
- Information by UniProt
- B-related factor 2 antibody
- BRF-2 antibody
- BRF2 antibody
ab17011 at 1.5
µg/ml detecting BRF2 from HeLa cell lysate (35ug total protein per lane) by Western blot. Primary antibody was incubated for 1 hour. The blot was detected by chemiluminescence.
ab17011 used in ChIP. For details please see Abreview.
This product has been referenced in:
- Gouge J et al. Redox Signaling by the RNA Polymerase III TFIIB-Related Factor Brf2. Cell 163:1375-87 (2015). Read more (PubMed: 26638071) »
- Lockwood WW et al. Integrative genomic analyses identify BRF2 as a novel lineage-specific oncogene in lung squamous cell carcinoma. PLoS Med 7:e1000315 (2010). WB ; Human . Read more (PubMed: 20668658) »