Anti-BRF2 antibody - N-terminal (ab194442)
Key features and details
- Mouse polyclonal to BRF2 - N-terminal
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-BRF2 antibody - N-terminal
See all BRF2 primary antibodies -
Description
Mouse polyclonal to BRF2 - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey -
Immunogen
Recombinant fragment (GST-tag) corresponding to Human BRF2 aa 1-100 (N terminal).
Database link: Q9HAW0 -
Positive control
- BRF2 recombinant protein; Human ovarian cancer tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab194442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2500. Predicted molecular weight: 47 kDa.
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Notes |
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WB
1/500 - 1/2500. Predicted molecular weight: 47 kDa. |
Target
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Function
General activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites. -
Sequence similarities
Belongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 512789 Cow
- Entrez Gene: 55290 Human
- Entrez Gene: 66653 Mouse
- Entrez Gene: 306542 Rat
- Omim: 607013 Human
- SwissProt: Q29S07 Cow
- SwissProt: Q4R318 Cynomolgus monkey
- SwissProt: Q9HAW0 Human
see all -
Alternative names
- B-related factor 2 antibody
- BRF-2 antibody
- BRF2 antibody
see all
Images
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Anti-BRF2 antibody - N-terminal (ab194442) at 1/2500 dilution + BRF2 recombinant protein (immunizing peptide) at 0.2 µg
Predicted band size: 47 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.
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Anti-BRF2 antibody - N-terminal (ab194442) at 1/1000 dilution + Human ovarian cancer tissue lysate at 50 µg
Predicted band size: 47 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab194442 has been referenced in 2 publications.
- Bian Y et al. Silencing of BRF2 inhibits the growth and metastasis of lung cancer cells. Mol Med Rep 22:1767-1774 (2020). PubMed: 32705258
- Wang L et al. MicroRNA-373 Inhibits Cell Proliferation and Invasion via Targeting BRF2 in Human Non-small Cell Lung Cancer A549 Cell Line. Cancer Res Treat 50:936-949 (2018). PubMed: 29025258