Key features and details
- Mouse polyclonal to BRF2 - N-terminal
- Suitable for: WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Product nameAnti-BRF2 antibody - N-terminal
See all BRF2 primary antibodies
DescriptionMouse polyclonal to BRF2 - N-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Cow, Cynomolgus monkey
- BRF2 recombinant protein; Human ovarian cancer tissue lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 50% Glycerol (glycerin, glycerine)
Concentration information loading...
Our Abpromise guarantee covers the use of ab194442 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2500. Predicted molecular weight: 47 kDa.|
FunctionGeneral activator of RNA polymerase III transcription. Factor exclusively required for RNA polymerase III transcription of genes with promoter elements upstream of the initiation sites.
Sequence similaritiesBelongs to the TFIIB family.
Contains 1 TFIIB-type zinc finger.
- Information by UniProt
- B-related factor 2 antibody
- BRF-2 antibody
- BRF2 antibody
Anti-BRF2 antibody - N-terminal (ab194442) at 1/2500 dilution + BRF2 recombinant protein (immunizing peptide) at 0.2 µg
Predicted band size: 47 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
The observed band size may not correspond to the predicted protein molecular weight as the immunogen (recombinant fragment) was used for the test lane.
Anti-BRF2 antibody - N-terminal (ab194442) at 1/1000 dilution + Human ovarian cancer tissue lysate at 50 µg
Predicted band size: 47 kDa
ab194442 has been referenced in 1 publication.
- Wang L et al. MicroRNA-373 Inhibits Cell Proliferation and Invasion via Targeting BRF2 in Human Non-small Cell Lung Cancer A549 Cell Line. Cancer Res Treat 50:936-949 (2018). PubMed: 29025258