Overview

  • Product name
  • Description
    Rabbit polyclonal to BRG1
  • Host species
    Rabbit
  • Specificity
    We have conflicting data about the performance of this antibody in ChIP. Publications have reported positive results with this antibody using ChIP application, however we also have customer data indicating certain batches of this antibody did not work in ChIP in their hands. If you require any further information or assistance please contact Abcam Scientific Support Team.
  • Tested applications
    Suitable for: IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1400 - 1500 of Human BRG1.

    Read Abcam's proprietary immunogen policy (Peptide available as ab13736.)

  • Positive control
    • This antibody gave a positive signal in the following cell lysate preparations: Jurkat; Jurkat nuclear; HepG2 nuclear.

Properties

Applications

Our Abpromise guarantee covers the use of ab4081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. PubMed: 20522713
WB 1/500 - 1/1000. Detects a band of approximately 235 kDa (predicted molecular weight: 185 kDa).
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
    • Tissue specificity
      Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
    • Involvement in disease
      Defects in SMARCA4 are the cause of rhabdoid tumor predisposition syndrome type 2 (RTPS2) [MIM:613325]. RTPS2 is a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood.
    • Sequence similarities
      Belongs to the SNF2/RAD54 helicase family.
      Contains 1 bromo domain.
      Contains 1 helicase ATP-binding domain.
      Contains 1 helicase C-terminal domain.
      Contains 1 HSA domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • ATP dependent helicase SMARCA4 antibody
      • ATP-dependent helicase SMARCA4 antibody
      • BAF 190 antibody
      • BAF190 antibody
      • BAF190A antibody
      • Brahma protein like 1 antibody
      • BRG1 antibody
      • BRG1 associated factor 190A antibody
      • BRG1 protein antibody
      • BRG1-associated factor 190A antibody
      • BRM/SWI2 related gene 1 antibody
      • Global transcription activator homologous sequence antibody
      • global transcription activator snf2l4 antibody
      • Homeotic gene regulator antibody
      • hSNF2b antibody
      • Mitotic growth and transcription activator antibody
      • MRD16 antibody
      • Nuclear protein GRB1 antibody
      • Protein brahma homolog 1 antibody
      • Protein BRG-1 antibody
      • Protein BRG1 antibody
      • RTPS2 antibody
      • SMARC A4 antibody
      • SMARCA4 antibody
      • SMCA4_HUMAN antibody
      • SNF2 antibody
      • SNF2 beta antibody
      • SNF2 like 4 antibody
      • SNF2-beta antibody
      • SNF2B antibody
      • SNF2L4 antibody
      • SNF2LB antibody
      • Sucrose nonfermenting like 4 antibody
      • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 antibody
      • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody
      • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 antibody
      • SWI2 antibody
      • Transcription activator BRG1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (40 µg)
      Lane 2: BRG1 knockout HAP1 cell lysate (40 µg)
      Lane 3: HeLa cell lysate (40 µg)
      Lane 4: K562 cell lysate (40 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab4081 observed at 240 kDa. Red - loading control, ab18058, observed at 124 kDa.

      ab4081 was shown to recognize BRG1 when BRG1 knockout samples were used, along with additional cross-reactive bands. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE. Ab4081 and ab18058 (loading control to Vinculin) were diluted at 1/500 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) with Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-BRG1 antibody (ab4081) at 1 µg/ml

      Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 2 : Jurkat nuclear extract lysate (ab14844)
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : HepG2 nuclear extract lysate (ab14660)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 185 kDa
      Observed band size: 235 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.

    • Rabbit polyclonal to BRG1 (ab4081) at 1/500 on HeLa Nuclear extract (20 ug per lane).

      Lane 1: ab4081 (1/500)
      Lane 2: ab4081 (1/500) + blocking peptide (1 ug)

      Secondary antibody: Goat anti-rabbit (HRP) - ab6721

    • Whole cell lysate prepared from HeLa cells was loaded at 150000 cells.
      ab4081 used at a 1/1000 dilution.
      The secondary used was an HRP conjugated donkey polyclonal used at a 1/5000 dilution.

      See Abreview

    References

    This product has been referenced in:
    • Del Gaudio N  et al. BRD9 binds cell type-specific chromatin regions regulating leukemic cell survival via STAT5 inhibition. Cell Death Dis 10:338 (2019). Read more (PubMed: 31000698) »
    • Gowda P  et al. Mutant Isocitrate Dehydrogenase 1 Disrupts PKM2-ß-Catenin-BRG1 Transcriptional Network-Driven CD47 Expression. Mol Cell Biol 38:N/A (2018). Read more (PubMed: 29463646) »
    See all 24 Publications for this product

    Customer reviews and Q&As

    11-20 of 26 Abreviews or Q&A

    Answer

    I am very pleased to hear you would like to accept our offer and test the products in rat tissues. Each code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Question
    Answer

    Thank you for confirming the batch and the order number. It is much appreciated.

    This is to let you know that I have just contacted and asked our Account Department to raise a credit note for you - for the cost of one vial of ab4081. For your information, the internal reference note for this credit is CN20325. You can use this credit in the near future for any of the products which are in the catalogue.

    We apologize for any inconvenience caused and thank you for bringing this matter to our attention.

    Read More

    Answer

    Thank you for your enquiry regarding ab4081.
    This is to confirm that the ChIP application has been removed very recently. As the datasheet indicates now - "Publications have reported positive results with this antibody in ChIP. However both our own and customer data has been variable."
    If you need any further assistance in the future, please do not hesitate to contact me.

    Read More

    Answer

    I am very sorry to hear that your customer is upset.

    The ChIP application has been removed very recently 19/3/2012 and I assume your customer purchased this product before this change.

    Publications have reported positive results with this antibody in ChIP. However both our own and customer data has been variable.

    We have received only a couple complaints (about one particular batch: GR37609-1 (AP775792) but keep monitoring the performance of this antibody. We have removed this application and added this note on the on-line datasheet for precaution.

    Could you please confirm the batch number and the Abcam Order number or your PON?

    Let me know how your customer would like to proceed with this enquiry.

    Read More

    Answer

    Thank you for your enquiry regarding ab4081.

    I would like to reassure you that our Abpromise applies to your complaint since you purchased this product within the guarantee period. This means that in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

    Could you please confirm the batch number and the Abcam Order number or your PON?

    I look forward to hearing from you and hope to solve this problem as soon as possible.

    .

    Read More

    Answer

    Thank you for your reply.

    I did an alignment between the immunogen sequence and the protein sequence from NM_001171953.1 and there is not a high enough match (only 67% homology) that I think the antibody ab4081 would work in Xenopus tissue.

    I would however recommend ab118558 as this antibody has beenpredicted to work in Xenopus tissue. Since it is only predicyed to work inXenopus laevis, I can offer a discount off a future purchase if you buy ab118558 now, test it inXenopuslaevisand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test ab118558 inXenopus laevis. I will then send a discount code. This code must be issued before purchasing ab118558 so please wait for my reply before ordering.

    2. Purchase ab118558 either by phone, fax, or online (www.abcam.com).

    3. Test it inXenopus laevis.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab118558 turns out to be unsuitable forXenopus laevis, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.


    Thank you for providing that information.

    Read More

    Answer

    Thank you for contacting Abcam.

    The immunogen sequence for ab4081 is proprietary so I cannot send you the sequence. However I did blast the immunogen sequence against the frog database but no hits came back.

    If you could send me the Xenopus sequence that you have been using, I will be more than happy to do an alignment and see if there is a chance that this antibody would work for you.

    I look forward to your reply.

    Read More
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa whole cell lysate)
    Loading amount
    150000 cells
    Specification
    HeLa whole cell lysate
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Feb 13 2012

    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - nuclear (HeLa)
    Total protein in input
    4e+006 cells
    Specification
    HeLa
    Immuno-precipitation step
    Other - anti-rabbit Dynabeads

    Abcam user community

    Verified customer

    Submitted Nov 10 2011

    Answer

    Thank you for contacting us. As discussed on phone we unfortunately do not sell small sizes of our products. The reason is that; we have over 70,000 products in our catalogue and having the small sized sample of each would be difficult to keep. However in order to help our customers we have extended our Abpromise guarantee. As per our further conversation if you could get in touch again after selecting few products then I will certainly look into your request for further advice. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    11-20 of 26 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up