Western blot abreview for Anti-BRG1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa whole cell lysate)
Loading amount
150000 cells
Specification
HeLa whole cell lysate
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% BSA in TBS

Secondary antibody

Name
Non-Abcam antibody was used: GE Healthcare NA9340V
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 225 kDa

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Submitted Feb 13 2012

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