Western blot abreview for Anti-BRG1 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
20 µg
Specification
HeLa cells
Treatment
siRNA transfections 40 hours
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 40 minute(s) · Concentration: 4% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in PBST

Secondary antibody

Name
Non-Abcam antibody was used: Jackson Goat-anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 200 kDa
Positive control
HeLa cells were treated with either control siRNA, or siRNA for BRG1 (third lane). As seen, there was a significant decrease in BRG1 protein level in siBRG1 treated cells, as visualized with these antibodies.

Dr. Efrat Shema

Verified customer

Submitted Apr 04 2012

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