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Human Cell lysate - nuclear (HeLa cells)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
I examined several cites that were reported to be occupied by BRG1, such as p53 and p21 promoters, CD44 transcriptional start site and transcribed region
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP buffer
The signal is very poor, only slightly above the background signal (determined by non-targeting control antibody). In most samples I got less than 0.01% of input.
The antibody is never tested in ChiP. We have no data to claim that the antibody will work in ChiP therefore we will welcome any feedback other customers have, regarding the ChiP application.
Dr. Efrat Shema
Submitted Apr 04 2012