ChIP abreview for Anti-BRG1 antibody

Poor
Abreviews
Application
ChIP
Sample
Human Cell lysate - nuclear (HeLa cells)
Specification
HeLa cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
I examined several cites that were reported to be occupied by BRG1, such as p53 and p21 promoters, CD44 transcriptional start site and transcribed region

Other product details

Dilution
1/60
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP buffer

Additional data

Additional Notes
The signal is very poor, only slightly above the background signal (determined by non-targeting control antibody). In most samples I got less than 0.01% of input.
Abcam response
The antibody is never tested in ChiP. We have no data to claim that the antibody will work in ChiP therefore we will welcome any feedback other customers have, regarding the ChiP application.

Dr. Efrat Shema

Verified customer

Submitted Apr 04 2012

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