Immunocytochemistry/ Immunofluorescence abreview for Anti-BRG1 antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS

Other product details

Dilution
1/200
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
Asynchronous HeLa cells were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a strong, nuclear-enriched, focal staining pattern. Methanol fixed samples were also evaluated and failed to produce a similar pattern.

Dr. Kirk Mcmanus

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Submitted Apr 18 2012

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