Given the amount of protein loaded, the signal is *very* weak. Other manufacturers have much more sensitive antibodies. I'm very disappointed with this antibody
I am sorry to hear that the antibody did not fill customer's expectations. We have not had any other complaints about this antibody. Unfortunately, we do not know if other antibodies were also rabbit polyclonals and if the same secondary antibody was used. Overnight incubation could be tried.
Abcam user community
Submitted May 06 2010
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