Western blot abreview for Anti-BRG1 antibody

Poor
Abreviews
Application
Western blot
Sample
Human Purified protein (Flag-purified Sf9-expressed human Brg1)
Loading amount
0.6 µg
Specification
Flag-purified Sf9-expressed human Brg1
Gel Running Conditions
Reduced Denaturing (7%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: PBST/1% BSA

Secondary antibody

Name
Non-Abcam antibody was used: donkey anti-rabbit HRP F(ab)2
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 220 kDa

Additional data

Additional Notes
Given the amount of protein loaded, the signal is *very* weak. Other manufacturers have much more sensitive antibodies. I'm very disappointed with this antibody
Abcam response
I am sorry to hear that the antibody did not fill customer's expectations.
We have not had any other complaints about this antibody.
Unfortunately, we do not know if other antibodies were also rabbit polyclonals and if the same secondary antibody was used.
Overnight incubation could be tried.

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Verified customer

Submitted May 06 2010

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