Recombinant Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-BRG1 antibody [EPNCIR111A] - BSA and Azide free
See all BRG1 primary antibodies -
DescriptionRabbit monoclonal [EPNCIR111A] to BRG1 - BSA and Azide free
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, Flow Cyt, ICC/IF, IP, ChIPmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Mouse BRG1 aa 200-300.
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Positive control
- K562, HeLa, MOLT4, NIH3T3, and PC12 cell lysates; Human kidney and testis tissues; HeLa cells.
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General notes
The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).
ab215998 is a PBS-only buffer formulated version of ab110641, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab110641 for information on protocols, dilutions, and image data.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Gordon Hager. View antibodies from NCI Center for Cancer Research Collaboration.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.20
Constituent: PBS -
Concentration information loading... -
PurityAffinity purified
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ClonalityMonoclonal
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Clone numberEPNCIR111A
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
- Anti-BRG1 antibody [EPNCIR111A] (ab110641)
- Anti-BRG1 antibody [EPNCIR111A] (Alexa Fluor® 488) (ab196314)
- Anti-BRG1 antibody [EPNCIR111A] (HRP) (ab196315)
- Anti-BRG1 antibody [EPNCIR111A] (Alexa Fluor® 647) (ab196535)
- Anti-BRG1 antibody [EPNCIR111A] (Alexa Fluor® 594) (ab207052)
- Anti-BRG1 antibody [EPNCIR111A] (Alexa Fluor® 568) (ab207055)
- Anti-BRG1 antibody [EPNCIR111A] (Phycoerythrin) (ab225124)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab215998 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IHC-P | Use at an assay dependent concentration. Antigen retrieval is recommended. Heat up to 98 °C, below boiling, and then let cool for 10-20 min. |
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 185 kDa. | |
| Flow Cyt | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. | |
| ChIP | Use at an assay dependent concentration. |
Target
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FunctionTranscriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
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Tissue specificityColocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
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Involvement in diseaseDefects in SMARCA4 are the cause of rhabdoid tumor predisposition syndrome type 2 (RTPS2) [MIM:613325]. RTPS2 is a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood.
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Sequence similaritiesBelongs to the SNF2/RAD54 helicase family.
Contains 1 bromo domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 HSA domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 6597 Human
- Entrez Gene: 20586 Mouse
- Entrez Gene: 171379 Rat
- Omim: 603254 Human
- SwissProt: P51532 Human
- SwissProt: Q3TKT4 Mouse
- SwissProt: Q8K1P7 Rat
- Unigene: 327527 Human
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Alternative names
- ATP dependent helicase SMARCA4 antibody
- ATP-dependent helicase SMARCA4 antibody
- BAF 190 antibody
see all
Images
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![ChIP - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)](https://www.abcam.com/ps/products/215/ab215998/Images/ab215998-295842-anti-brg1-antibody-epncir111a-chip.jpg)
ChIP - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)This ChIP data was generated using the same anti-BRG1 antibody clone, EPNCIR111A, in a different buffer formulation (cat# ab110641).
ChIP analysis using ab110641 binding BRG1 in mouse bone marrow derived macrophages. Cells were cross-linked for 10 minutes with formaldehyde. Samples were incubated with undiluted primary antibody for 16 hours at 4°C. Protein binding was detected using real-time PCR.
Positive control: PU.1 antibody.
Negative Control: rabbit IgG. -
![Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)](https://www.abcam.com/ps/products/215/ab215998/Images/ab215998-327135-anti-brg1-antibody-epncir111a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)ab110641 staining BRG1 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab110641 at 1/500 dilution and ab195889 (Mouse monoclonal [DM1A] to alpha Tubulin - Microtubule Marker (Alexa Fluor® 594)) at 1/250 dilution overnight at +4°C, followed by a further incubation at room temperature for 1h with ab150081 (Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488)) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab110641).
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![Flow Cytometry - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)](https://www.abcam.com/ps/products/215/ab215998/Images/ab215998-327134-anti-brg1-antibody-epncir111a-flow-cytometry.jpg)
Flow Cytometry - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling BRG1 with purified ab110641 at 1/200 dilution(10µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab110641).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)](https://www.abcam.com/ps/products/215/ab215998/Images/ab215998-327133-anti-brg1-antibody-epncir111a-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998) -
![Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)](https://www.abcam.com/ps/products/215/ab215998/Images/ab215998-295841-anti-brg1-antibody-epncir111a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)This ICC/IF data was generated using the same anti-BRG1 antibody clone, EPNCIR111A, in a different buffer formulation (cat# ab110641).
ab110641 staining BRG1 in wild-type HAP1 cells (top panel) and BRG1 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab110641 at 1/500 dilution and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)](https://www.abcam.com/ps/products/215/ab215998/Images/ab215998-295840-anti-brg1-antibody-epncir111a-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPNCIR111A] - BSA and Azide free (ab215998)
Protocols
Datasheets and documents
References
This product has been referenced in:
- Otte A et al. In vitro and in vivo therapeutic approach for a small cell carcinoma of the ovary hypercalcaemic type using a SCCOHT-1 cellular model. Orphanet J Rare Dis 9:126 (2014). WB ; Human . Read more (PubMed: 25103190) »
- Goljanek-Whysall K et al. myomiR-dependent switching of BAF60 variant incorporation into Brg1 chromatin remodeling complexes during embryo myogenesis. Development 141:3378-87 (2014). WB ; Mouse . Read more (PubMed: 25078649) »
