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  1. Link

    brg1-antibody-epr3912-ab108318.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-BRG1 antibody [EPR3912] (ab108318)

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  • SDS
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Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Flow Cytometry (Intracellular) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
  • Anti-BRG1 antibody [EPR3912] (ab108318)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3912] to BRG1
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-BRG1 antibody [EPR3912]
    See all BRG1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3912] to BRG1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1, HEK-293T, K562, RAW264.7, NIH/3T3, PC-12 and Molt-4 cell lysate. ICC/IF: HeLa cells; SMARCA4-HAP1 cells. IHC-P: Human colon, urinary bladder transitional carcinoma, breast carcinoma, bladder cancer, ovarian carcinoma and normal tonsil tissue, mouse and rat stomach tissue. Flow Cyt (intra): HeLa cells
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3912
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Swi / Snf
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Nuclear
    • Epigenetics and Nuclear Signaling
    • Chromatin Remodeling
    • Trithorax
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-BRG1 antibody [EPR3912] (ab206598)
    • Alexa Fluor® 488 Anti-BRG1 antibody [EPR3912] (ab206712)
    • Anti-BRG1 antibody [EPR3912] - BSA and Azide free (ab222230)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human SMARCA4 (BRG1) knockout HEK-293T cell line (ab255432)
  • KO cell lysates

    • Human SMARCA4 (BRG1) knockout HEK-293T cell lysate (ab263853)
  • KO cell pellets

    • Human SMARCA4 (BRG1) knockout HEK-293T cell pellet (ab278821)
  • Positive Controls

    • K-562 nuclear extract lysate (ab14851)
  • Recombinant Protein

    • Recombinant human BRG1 protein (ab82237)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.

For unpurified format use at 1/500 dilution.

WB (2)
1/1000 - 1/5000. Predicted molecular weight: 185 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

Flow Cyt (Intra)
1/20.
Notes
ICC/IF
1/50.

For unpurified format use at 1/500 dilution.

WB
1/1000 - 1/5000. Predicted molecular weight: 185 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.

Flow Cyt (Intra)
1/20.

Target

  • Function

    Transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1.
  • Tissue specificity

    Colocalizes with ZEB1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level).
  • Involvement in disease

    Defects in SMARCA4 are the cause of rhabdoid tumor predisposition syndrome type 2 (RTPS2) [MIM:613325]. RTPS2 is a familial cancer syndrome predisposing to renal or extrarenal malignant rhabdoid tumors and to a variety of tumors of the central nervous system, including choroid plexus carcinoma, medulloblastoma, and central primitive neuroectodermal tumors. Rhabdoid tumors are the most aggressive and lethal malignancies occurring in early childhood.
  • Sequence similarities

    Belongs to the SNF2/RAD54 helicase family.
    Contains 1 bromo domain.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
    Contains 1 HSA domain.
  • Post-translational
    modifications

    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P51532 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6597 Human
    • Entrez Gene: 20586 Mouse
    • Entrez Gene: 171379 Rat
    • Omim: 603254 Human
    • SwissProt: P51532 Human
    • SwissProt: Q3TKT4 Mouse
    • SwissProt: Q8K1P7 Rat
    • Unigene: 327527 Human
    • Unigene: 286593 Mouse
    • Unigene: 23417 Rat
    see all
  • Alternative names

    • ATP dependent helicase SMARCA4 antibody
    • ATP-dependent helicase SMARCA4 antibody
    • BAF 190 antibody
    • BAF190 antibody
    • BAF190A antibody
    • Brahma protein like 1 antibody
    • BRG1 antibody
    • BRG1 associated factor 190A antibody
    • BRG1 protein antibody
    • BRG1-associated factor 190A antibody
    • BRM/SWI2 related gene 1 antibody
    • Global transcription activator homologous sequence antibody
    • global transcription activator snf2l4 antibody
    • Homeotic gene regulator antibody
    • hSNF2b antibody
    • Mitotic growth and transcription activator antibody
    • MRD16 antibody
    • Nuclear protein GRB1 antibody
    • Protein brahma homolog 1 antibody
    • Protein BRG-1 antibody
    • Protein BRG1 antibody
    • RTPS2 antibody
    • SMARC A4 antibody
    • SMARCA4 antibody
    • SMCA4_HUMAN antibody
    • SNF2 antibody
    • SNF2 beta antibody
    • SNF2 like 4 antibody
    • SNF2-beta antibody
    • SNF2B antibody
    • SNF2L4 antibody
    • SNF2LB antibody
    • Sucrose nonfermenting like 4 antibody
    • SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily A member 4 antibody
    • SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4 antibody
    • SWI2 antibody
    • Transcription activator BRG1 antibody
    see all

Images

  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    All lanes : Anti-BRG1 antibody [EPR3912] (ab108318) at 1/10000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : SMARCA4 knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 185 kDa
    Observed band size: 185 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab108318 observed at 185 kDa. Red - Anti-Vinculin antibody [VIN-54] observed at 124 kDa.

     ab108318 was shown to react with SMARCA4 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab255432 (knockout cell lysate ab263853) was used. Wild-type HEK-293T and SMARCA4 knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab108318 and Anti-Vinculin antibody [VIN-54] overnight at 4°C at a 1 in 10000 Dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human bladder cancer tissue sections labeling BRG1 with purified ab108318 at 1/500 dilution (0.23 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    All lanes : Anti-BRG1 antibody [EPR3912] (ab108318) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : BRG1 knockout HAP1 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : Molt-4 cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 185 kDa
    Additional bands at: 185 kDa. We are unsure as to the identity of these extra bands.



    Lanes 1 - 4: Merged signal (red and green). Red - loading control, ab18058 (unpurified), observed at 124 kDa.

    ab108318 was shown to specifically react with BRG1 in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when BRG1 knockout samples were used. Wild-type and BRG1 knockout samples were subjected to SDS-PAGE, ab108318 and ab18058 (loading control to Vinculin) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1hr at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BRG1 with purified ab108318 at 1/50 dilution (2.3 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Flow Cytometry (Intracellular) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BRG1 with purified ab108318 at 1/20 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). 

     

     

  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    Different batches of ab108318 were tested on K-562 (Human chronic myelogenous leukemia lymphoblast) lysate at 0.05 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 185 kDa.
  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    All lanes : Anti-BRG1 antibody [EPR3912] (ab108318) at 1/1000 dilution (Purified)

    Lane 1 : K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysates
    Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 185 kDa
    Observed band size: 185 kDa

  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    Anti-BRG1 antibody [EPR3912] (ab108318) at 1/10000 dilution (Purified) + RAW264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 185 kDa
    Observed band size: 185 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat stomach tissue sections labeling BRG1 with purified ab108318 at 1/500 dilution (0.23 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse stomach tissue sections labeling BRG1 with purified ab108318 at 1/500 dilution (0.23 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)

    ab108318 (unpurified) staining BRG1 in wild-type HAP1 cells (top panel) and BRG1 knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab108318 at 1/500 dilution and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Formalin-fixed paraffin-embedded human colon tissue stained for BRG1 using ab108318 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    Western blot - Anti-BRG1 antibody [EPR3912] (ab108318)
    All lanes : Anti-BRG1 antibody [EPR3912] (ab108318) at 1/1000 dilution (unpurified)

    Lane 1 : K562 cell lysate
    Lane 2 : Molt-4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 185 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunocytochemistry/ Immunofluorescence - Anti-BRG1 antibody [EPR3912] (ab108318)

    ab108318 (unpurified) staining BRG1 in HeLa cells. The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab108318 at 1/500 dilution and ab195889 at 1/250 dilution (shown in pseudocolour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Formalin-fixed paraffin-embedded human Urinary bladder transitional carcinoma tissue.stained for BRG1 using ab108318 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Formalin-fixed paraffin-embedded human Urinary Breast carcinoma tissue stained for BRG1 using ab108318 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Formalin-fixed paraffin-embedded human Ovarian carcinoma tissue stained for BRG1 using ab108318 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRG1 antibody [EPR3912] (ab108318)

    Formalin-fixed paraffin-embedded Normal human tonsil tissue stained for BRG1 using ab108318 at 1/100 dilution in immunohistochemical analysis. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Anti-BRG1 antibody [EPR3912] (ab108318)
    Anti-BRG1 antibody [EPR3912] (ab108318)

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (5)

Publishing research using ab108318? Please let us know so that we can cite the reference in this datasheet.

ab108318 has been referenced in 5 publications.

  • Lin DI  et al. SMARCA4 inactivation defines a subset of undifferentiated uterine sarcomas with rhabdoid and small cell features and germline mutation association. Mod Pathol 32:1675-1687 (2019). PubMed: 31190001
  • Farnaby W  et al. BAF complex vulnerabilities in cancer demonstrated via structure-based PROTAC design. Nat Chem Biol 15:672-680 (2019). PubMed: 31178587
  • Dong Y  et al. HOXC-AS1-MYC regulatory loop contributes to the growth and metastasis in gastric cancer. J Exp Clin Cancer Res 38:502 (2019). PubMed: 31870402
  • Dudek AH  et al. Partial Inactivation of the Chromatin Remodelers SMARCA2 and SMARCA4 in Virus-Infected Cells by Caspase-Mediated Cleavage. J Virol 92:N/A (2018). PubMed: 29848589
  • Vangamudi B  et al. The SMARCA2/4 ATPase Domain Surpasses the Bromodomain as a Drug Target in SWI/SNF-Mutant Cancers: Insights from cDNA Rescue and PFI-3 Inhibitor Studies. Cancer Res 75:3865-78 (2015). PubMed: 26139243

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-BRG1 antibody [EPR3912]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T and Xenopus laevis oocyte)
Gel Running Conditions
Reduced Denaturing (10% SDS)
Loading amount
10 µg
Specification
HEK293T and Xenopus laevis oocyte
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Andy Bannister

Verified customer

Submitted May 12 2021

Western blot abreview for Anti-BRG1 antibody [EPR3912]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Oocytes stage V)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
1 cells
Specification
Oocytes stage V
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 03 2020

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