Overview

  • Product name

    Anti-BRI3 antibody
  • Description

    Rabbit polyclonal to BRI3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse, Cow, Pig
  • Immunogen

    Recombinant fragment corresponding to Human BRI3 aa 110-258.

  • Positive control

    • HeLa, HepG2, MCF7 cell lines.
  • General notes

    Protein previously labeled as ITM2C.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 30 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use 10mM Citrate buffer or Tris-EDTA buffer (PH8.0)

Target

  • Function

    Negative regulator of beta amyloid peptide production. May inhibit the processing of APP by blocking its access to alpha-and beta-secretase. Binding to the beta-secretase-cleaved APP C-terminal fragment is negligible, suggesting that ITM2C is a poor gamma-secretase cleavage inhibitor. May play a role in TNF-induced cell death and neuronal differentiation.
  • Tissue specificity

    High levels in the brain, specifically in the cerebral cortex, medulla, amygdala, hippocampus, thalamus, caudate nucleus, cerebellum, olfactory lobe and spinal cord. Very low levels in other organs.
  • Sequence similarities

    Belongs to the ITM2 family.
    Contains 1 BRICHOS domain.
  • Post-translational
    modifications

    Type I membrane-bound, as well as soluble, furin has a pre-eminent role in ITM2C proteolytic processing. PCSK7 and PCSK5 may also be involved although to a lesser extent. The soluble form of PCSK7 is incapable of processing ITM2C.
  • Cellular localization

    Lysosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • BRI3 antibody
    • BRICD2C antibody
    • BRICHOS domain containing 2C antibody
    • Cerebral protein 14 antibody
    • CT-BRI3 antibody
    • E25 antibody
    • E25C antibody
    • Integral membrane protein 2C antibody
    • Integral membrane protein 3 antibody
    • Itm2c antibody
    • ITM2C_HUMAN antibody
    • ITM3 antibody
    • NPD018 antibody
    • Transmembrane protein BRI3 antibody
    see all

Images

  • Anti-BRI3 antibody (ab101389) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 30 kDa



    12% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using ab101389 at 1/100 dilution.

References

This product has been referenced in:

  • Dolfe L  et al. The Bri2 and Bri3 BRICHOS Domains Interact Differently with Aß42 and Alzheimer Amyloid Plaques. J Alzheimers Dis Rep 2:27-39 (2018). Read more (PubMed: 30480246) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (SH-SY5Y)
Total protein in input
1000 µg
Immuno-precipitation step
Other - Dynabeads
Specification
SH-SY5Y

Ms. Filipa Martins

Verified customer

Submitted Jun 20 2014

Application
Western blot
Sample
Rat Tissue lysate - whole (Cortex)
Gel Running Conditions
Reduced Denaturing (5-20% gradient)
Loading amount
100 µg
Specification
Cortex
Blocking step
Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Ms. Filipa Martins

Verified customer

Submitted Nov 04 2013

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