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Dear Madam / Sir, A customer would like to buy a mounting medium to use IHC and IF. Which ABCAM mounting medium can be used with both of these applications? Thank you in advance.
Asked on Jan 25 2012
Thank you for contacting us.
Differences in the chemical compositions of the mounting medium make using one product for both IHC and IF difficult. The conditions required for excellent perioxidase preservation are not neccessarily good for the anti- photobleaching requirements of IF. As such I cannot recommend a single medium for you customer but suggest they by mounting mediums specific to their needs.
Our standard mointing medium (ab64230;https://www.abcam.com/Mounting-Medium-ab64230.html) is appropriate for IHC.
For IF I recommend BrightMount/Plus (ab103748;https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html)
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Answered on Jan 25 2012