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We have a client with some questions about the Easylink kits. After the conjugation of the antibodies and the final mounting of the slide, how much time the fluorescence stands able to be seen in the microscopy before fading? A day? A week? How much time is it recomendable to keep the slide under the light of the microscope? Is there any difference between the time of the fluorescences fading? He is a costumer that demands a really deep theoretical basis before make any purchase.
Asked on Sep 26 2012
Thank you for your inquiry.
The supplying lab of our EasyLink kits doesn't carry out microscopy, so they haven't testing this themselves.Theyresearched AMCA’s degree of stability against photobleaching/fading, and confusingly different sources say different things: that it is resistant to photobleaching, that it has moderate photobleaching, or that it fades rapidly; that it retains its fluorescence much longer than fluorescein, or that fades as rapidly as fluorescein. Many sources do, however, recommend that an anti-fading agent is used with AMCA. Thereare a variety of anti-fading agents available, so you could say that the exact rate of fading will depend on the particular conditions selected.
We carry anti-fading mounting media as ab103748. https://www.abcam.com/BrightMount-Plus-25ml-Aqueous-Mounting-Medium-Anti-fading-for-Fluorescent-Staining-ab103748.html
I hope that helps. I apologize thatI have not been able to provide a more definite answer in this case.
Answered on Sep 26 2012