Brilliant Cresyl Blue Solution (1.5%) (ab246814)

Overview

  • Product name

    Brilliant Cresyl Blue Solution (1.5%)
    See all Reticulocytes reagents
  • General notes

    Brilliant Cresyl Blue Solution (1.5%) ab246814 is a supravital hematology stain for counting reticulocytes in peripheral blood smears. The number of reticulocytes in the blood is a simple measure of erythropoietic performance. Reticulocytes are immature erythrocytes that contain nuclear remnants of basophilic ribonucleoproteins which decreases as the reticulocyte matures. When stained, reticulocytes appear to contain dark blue granules or filaments.

    Staining Interpretation:

    Reticulocytes: Blue/Green with Dark Blue Granules.

    Erythrocytes: Blue/Green.

    Leukocytes: Light Blue.

    Control Tissue: Blood.

    Other products for staining tissue sections

    Find more kits and reagents in the special stains guide, or products for antigen retrieval, blocking, signal amplification, visualization, counterstaining, and mounting in the IHC kits and reagents guide.

    Procedure (Glass Tube): 

    1. Pipette a volume of Brilliant Cresyl Blue in the bottom of a glass tube followed by an equal volume of uncoagulated blood and mix well.

    2. Incubate mixture for 5-15 minutes in a warm water bath.

    3. Mix well prior to preparing smear.

    4. Prepare 2-3 glass slide smears and let dry.

    5. Clear slide and mount in synthetic resin.

    Procedure (Glass Slide): 

    1. Pipette a volume of Brilliant Cresyl Blue in the bottom of a glass slide followed by an equal volume of uncoagulated blood and mix well.

    2. Place immediately in a warm humidity chamber and incubate mixture for 5-15 minutes.

    3. Mix well prior to preparing smear.

    4. Prepare smear and let dry.

    5. Clear slide and mount in synthetic resin.

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at Room Temperature. Store at Room Temperature.
  • Storage buffer

    Constituents: 0.85% Sodium chloride, 1.5% Brilliant Cresyl Blue, 0.1% Safranin O
  • Concentration information loading...

Images

  • ab246814 Brilliant Cresyl Blue Solution (1.5%) staining reticulocytes (Blue/Green with Dark Blue Granules); Erythrocytes (Blue/Green) and Leukocytes (Light Blue). 

References

ab246814 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab246814.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up