Overview

  • Product name

    Anti-Brk/PTK6 (phospho Y447) antibody
    See all Brk/PTK6 primary antibodies
  • Description

    Rabbit polyclonal to Brk/PTK6 (phospho Y447)
  • Host species

    Rabbit
  • Specificity

    ab138368 detects endogenous levels of Brk/PTK6 only when phosphorylated at tyrosine 447

  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human Brk/PTK6 (C terminal).

  • Positive control

    • Extract of 293 cells treated with EGF
  • General notes

     This product was previously labelled as Brk

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS is without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab138368 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab138368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 52 kDa.

Target

  • Function

    Phosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
  • Tissue specificity

    Epithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Autophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
  • Cellular localization

    Cytoplasm. Nucleus. Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
  • Information by UniProt
  • Database links

  • Alternative names

    • Breast tumor kinase antibody
    • Breast tumour kinase antibody
    • Protein tyrosine kinase 6 antibody
    • Protein-tyrosine kinase 6 antibody
    • Ptk6 antibody
    • PTK6_HUMAN antibody
    • Tyrosine protein kinase BRK antibody
    • Tyrosine-protein kinase BRK antibody
    see all

Images

  • Anti-Brk/PTK6 (phospho Y447) antibody (ab138368) at 1/500 dilution + 293 cell extract treated with EGF 200ng/ml for 30 min at 30 µg

    Predicted band size: 52 kDa

References

This product has been referenced in:

  • Wozniak DJ  et al. PTEN is a protein phosphatase that targets active PTK6 and inhibits PTK6 oncogenic signaling in prostate cancer. Nat Commun 8:1508 (2017). Read more (PubMed: 29142193) »
  • Mathur PS  et al. Kinase-Dependent and -Independent Roles for PTK6 in Colon Cancer. Mol Cancer Res 14:563-73 (2016). Read more (PubMed: 26983689) »
See all 2 Publications for this product

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