Key features and details
- Rabbit polyclonal to Brk/PTK6 (phospho Y447)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Brk/PTK6 (phospho Y447) antibody
See all Brk/PTK6 primary antibodies
DescriptionRabbit polyclonal to Brk/PTK6 (phospho Y447)
ab138368 detects endogenous levels of Brk/PTK6 only when phosphorylated at tyrosine 447
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human Brk/PTK6 (C terminal).
- Extract of 293 cells treated with EGF
This product was previously labelled as Brk
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab138368 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Our Abpromise guarantee covers the use of ab138368 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 52 kDa.|
FunctionPhosphorylates KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. May function as an intracellular signal transducer in epithelial tissues. Overexpression in mammary cells leads to mitogenically sensitization to EGF, and results in a partially transformed phenotype. Its presence in the nucleus appears to be linked to suppression of tumor progression.
Tissue specificityEpithelia-specific. Very high level in colon and high levels in small intestine and prostate, and low levels in some fetal tissues. Expressed at low level in some breast tumors, but not in normal breast. Also found in melanocytes. Not expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Sequence similaritiesBelongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain.
modificationsAutophosphorylated. The phosphorylation of Tyr-447 may lead to the autoinhibition of the enzyme.
Cellular localizationCytoplasm. Nucleus. Colocalizes with KHDRBS1, KHDRBS2 or KHDRBS3, within the nucleus. In secretory epithelial cells from prostate adenocarcinoma, nuclear localization is higher in low-grade and lower in high-grade regions of the tumors.
- Information by UniProt
- Breast tumor kinase antibody
- Breast tumour kinase antibody
- Protein tyrosine kinase 6 antibody
ab138368 has been referenced in 2 publications.
- Wozniak DJ et al. PTEN is a protein phosphatase that targets active PTK6 and inhibits PTK6 oncogenic signaling in prostate cancer. Nat Commun 8:1508 (2017). PubMed: 29142193
- Mathur PS et al. Kinase-Dependent and -Independent Roles for PTK6 in Colon Cancer. Mol Cancer Res 14:563-73 (2016). PubMed: 26983689