Product nameAnti-BRMS1 antibody [EPR7202]
See all BRMS1 primary antibodies
DescriptionRabbit monoclonal [EPR7202] to BRMS1
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Human
Synthetic peptide within Human BRMS1 (N terminal). The exact sequence is proprietary.
- HeLa, 293T, HepG2, and Human colon cancer lysates; Human colon and placenta tissues; HeLa cells.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||1/50 - 1/100.|
|ICC/IF||1/250 - 1/500.|
|IP||1/10 - 1/100.|
FunctionTranscriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
Tissue specificityExpression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
Sequence similaritiesBelongs to the BRMS1 family.
Cellular localizationNucleus. Cytoplasm. Predominantly nuclear.
- Information by UniProt
- AV003220 antibody
- AW554636 antibody
- Breast cancer metastasis suppressor 1 antibody
All lanes : Anti-BRMS1 antibody [EPR7202] (ab134968) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human colon cancer lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Immunofluorescence staining of HepG2 cells with purified ab134968 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling BRMS1 with ab134968 at 1/50 dilution.
Imunohistochemical analysis of paraffin-embedded Human placenta tissue labelling BRMS1 with ab134968 at 1/50 dilution.
Immunohistochemical analysis using ab134968 showing positive staining in Ovarian carcinoma tissue.
Immunohistochemical analysis using ab134968 showing positive staining in Breast carcinoma tissue.
This product has been referenced in:
- Bucciarelli PR et al. BRMS1 Expression in Surgically Resected Lung Adenocarcinoma Predicts Future Metastases and Is Associated with a Poor Prognosis. J Thorac Oncol 13:73-84 (2018). IHC-P . Read more (PubMed: 29097253) »
- Kramer D et al. A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity. Cell Death Dis 6:e1634 (2015). Read more (PubMed: 25675294) »