Overview

  • Product name

    Anti-BRMS1 antibody [EPR7202]
    See all BRMS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7202] to BRMS1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BRMS1 (N terminal). The exact sequence is proprietary.

  • Positive control

    • HeLa, 293T, HepG2, and Human colon cancer lysates; Human colon and placenta tissues; HeLa cells.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7202
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab134968 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
IHC-P 1/50 - 1/100.
ICC/IF 1/250 - 1/500.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
    • Tissue specificity

      Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
    • Sequence similarities

      Belongs to the BRMS1 family.
    • Cellular localization

      Nucleus. Cytoplasm. Predominantly nuclear.
    • Information by UniProt
    • Database links

    • Alternative names

      • AV003220 antibody
      • AW554636 antibody
      • Breast cancer metastasis suppressor 1 antibody
      • Breast cancer metastasis-suppressor 1 antibody
      • BRMS1 antibody
      • BRMS1_HUMAN antibody
      • DKFZp564A063 antibody
      • MGC95128 antibody
      see all

    Images

    • All lanes : Anti-BRMS1 antibody [EPR7202] (ab134968) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 28 kDa
      Observed band size: 28 kDa

    • Immunofluorescence staining of HepG2 cells with purified ab134968 at a working dilution of 1/1000, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling BRMS1 with ab134968 at 1/50 dilution.
    • Imunohistochemical analysis of paraffin-embedded Human placenta tissue labelling BRMS1 with ab134968 at 1/50 dilution.
    • Immunohistochemical analysis using ab134968 showing positive staining in Ovarian carcinoma tissue.

    • Immunohistochemical analysis using ab134968 showing positive staining in Breast carcinoma tissue.

    References

    This product has been referenced in:

    • Bucciarelli PR  et al. BRMS1 Expression in Surgically Resected Lung Adenocarcinoma Predicts Future Metastases and Is Associated with a Poor Prognosis. J Thorac Oncol 13:73-84 (2018). IHC-P . Read more (PubMed: 29097253) »
    • Kramer D  et al. A pro-apoptotic function of iASPP by stabilizing p300 and CBP through inhibition of BRMS1 E3 ubiquitin ligase activity. Cell Death Dis 6:e1634 (2015). Read more (PubMed: 25675294) »
    See all 8 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134968.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up