Overview

  • Product name

    Anti-BRMS1 antibody [EPR7202] (HRP)
    See all BRMS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7202] to BRMS1 (HRP)
  • Host species

    Rabbit
  • Conjugation

    HRP
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human BRMS1 aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: Q9HCU9

  • Positive control

    • WB: HeLa whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
  • Storage buffer

    pH: 7.4
    Preservative: 0.1% Proclin
    Constituents: PBS, 1% BSA, 30% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR7202
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab208862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 33 kDa (predicted molecular weight: 28 kDa).

Target

  • Function

    Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down-regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma.
  • Tissue specificity

    Expression levels are higher in term placentas than in early placentas. Low levels of expression observed in normal pregnancies and in molar pregnancies.
  • Sequence similarities

    Belongs to the BRMS1 family.
  • Cellular localization

    Nucleus. Cytoplasm. Predominantly nuclear.
  • Information by UniProt
  • Database links

  • Alternative names

    • AV003220 antibody
    • AW554636 antibody
    • Breast cancer metastasis suppressor 1 antibody
    • Breast cancer metastasis-suppressor 1 antibody
    • BRMS1 antibody
    • BRMS1_HUMAN antibody
    • DKFZp564A063 antibody
    • MGC95128 antibody
    see all

Images

  • Anti-BRMS1 antibody [EPR7202] (HRP) (ab208862) at 1/5000 dilution + HeLa whole cell lysate (ab150035) at 10 µg

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 28 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab208862 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab208862 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab208862.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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