Anti-Brn-2 antibody (ab101726)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Brn-2 antibody
See all Brn-2 primary antibodies -
Description
Goat polyclonal to Brn-2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Pig
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Immunogen
Synthetic peptide corresponding to Human Brn-2 aa 37-52 (internal sequence).
Sequence:C-AQSLVQGDYGALQSN
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Positive control
- Purchase matching WB positive control:Recombinant Human Brn-2 protein
- WB: Human brain (cerebellum), mouse and rat brain tissue lysates.
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General notes
Protein name formally labeled as POU3F2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab101726 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab101726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa). |
Target
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Function
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. -
Tissue specificity
Expressed specifically in the neuroectodermal cell lineage. -
Sequence similarities
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 783320 Cow
- Entrez Gene: 5454 Human
- Entrez Gene: 18992 Mouse
- Entrez Gene: 29588 Rat
- Omim: 600494 Human
- SwissProt: P20265 Human
- SwissProt: P31360 Mouse
- SwissProt: P56222 Rat
see all -
Alternative names
- Brain 2 antibody
- Brain specific homeobox/POU domain protein 2 antibody
- Brain-2 antibody
see all
Images
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Western blot - Anti-Brn-2 antibody (ab101726)Anti-Brn-2 antibody (ab101726) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 47 kDa
Primary incubation was 1 hour.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Sakaeda M et al. Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer. Lab Invest : (2013). Read more (PubMed: 23358112) »
