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    brn-2-antibody-ab101726.pdf

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Anti-Brn-2 antibody (ab101726)

  • Datasheet
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Western blot - Anti-Brn-2 antibody (ab101726)

    Key features and details

    • Goat polyclonal to Brn-2
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    View more associated products

    Overview

    • Product name

      Anti-Brn-2 antibody
      See all Brn-2 primary antibodies
    • Description

      Goat polyclonal to Brn-2
    • Host species

      Goat
    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Human Brn-2 aa 37-52 (internal sequence).
      Sequence:

      C-AQSLVQGDYGALQSN

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Purchase matching WB positive control:Recombinant Human Brn-2 protein
      • WB: Human brain (cerebellum), mouse and rat brain tissue lysates.
    • General notes

      Protein name formally labeled as POU3F2.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      ab101726 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Neuroscience
      • Neurology process
      • Notch Pathway
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Domain Families
      • Developmental Families
      • POU
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • Mouse brain tissue lysate - nuclear extract (ab14649)
    • Recombinant Protein

      • Recombinant Human Brn-2 protein (ab134864)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab101726 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    All applications
    Cow
    Pig
    Application Abreviews Notes
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).

    1 hour primary incubation is recommended for this product.

    Notes
    WB
    Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 47 kDa).

    1 hour primary incubation is recommended for this product.

    Target

    • Function

      Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
    • Tissue specificity

      Expressed specifically in the neuroectodermal cell lineage.
    • Sequence similarities

      Belongs to the POU transcription factor family. Class-3 subfamily.
      Contains 1 homeobox DNA-binding domain.
      Contains 1 POU-specific domain.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession P20265 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 783320 Cow
      • Entrez Gene: 18992 Mouse
      • SwissProt: P31360 Mouse
      • Unigene: 389520 Mouse
      • Alternative names

        • Brain 2 antibody
        • Brain specific homeobox/POU domain protein 2 antibody
        • Brain-2 antibody
        • Brain-specific homeobox/POU domain protein 2 antibody
        • BRN 2 antibody
        • Brn-2 antibody
        • BRN2 antibody
        • N Oct 3 antibody
        • N OCT 3 Gene antibody
        • N Oct3 antibody
        • Nervous system specific octamer binding transcription factor antibody
        • Nervous system-specific octamer-binding transcription factor N-Oct-3 antibody
        • OCT 7 antibody
        • Oct-7 antibody
        • OCT7 antibody
        • Octamer Binding Transcription Factor 7 antibody
        • Octamer-binding protein 7 antibody
        • Octamer-binding transcription factor 7 antibody
        • OTF 7 antibody
        • OTF-7 antibody
        • OTF7 antibody
        • PO3F2_HUMAN antibody
        • POU class 3 homeobox 2, antibody
        • POU domain class 3 transcription factor 2 antibody
        • POU domain, class 3, transcription factor 2 antibody
        • Pou3f2 antibody
        • POUF 3 antibody
        • POUF3 antibody
        • Protein Brn 2 antibody
        see all

      Images

      • Western blot - Anti-Brn-2 antibody (ab101726)
        Western blot - Anti-Brn-2 antibody (ab101726)
        Anti-Brn-2 antibody (ab101726) at 0.1 µg/ml + Mouse brain lysate in RIPA buffer at 35 µg

        Developed using the ECL technique.

        Predicted band size: 47 kDa



        Primary incubation was 1 hour.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab101726? Please let us know so that we can cite the reference in this datasheet.

      ab101726 has been referenced in 1 publication.

      • Sakaeda M  et al. Neural lineage-specific homeoprotein BRN2 is directly involved in TTF1 expression in small-cell lung cancer. Lab Invest : (2013). PubMed: 23358112

      Customer reviews and Q&As

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