Overview

  • Product name
  • Description
    Rabbit polyclonal to Brn-2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment corresponding to Human Brn-2 aa 248-443.

  • Positive control
  • General notes

    Protein name formally labeled as POU3F2.

Properties

Applications

Our Abpromise guarantee covers the use of ab137469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
  • Tissue specificity
    Expressed specifically in the neuroectodermal cell lineage.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain 2 antibody
    • Brain specific homeobox/POU domain protein 2 antibody
    • Brain-2 antibody
    • Brain-specific homeobox/POU domain protein 2 antibody
    • BRN 2 antibody
    • Brn-2 antibody
    • BRN2 antibody
    • N Oct 3 antibody
    • N OCT 3 Gene antibody
    • N Oct3 antibody
    • Nervous system specific octamer binding transcription factor antibody
    • Nervous system-specific octamer-binding transcription factor N-Oct-3 antibody
    • OCT 7 antibody
    • Oct-7 antibody
    • OCT7 antibody
    • Octamer Binding Transcription Factor 7 antibody
    • Octamer-binding protein 7 antibody
    • Octamer-binding transcription factor 7 antibody
    • OTF 7 antibody
    • OTF-7 antibody
    • OTF7 antibody
    • PO3F2_HUMAN antibody
    • POU class 3 homeobox 2, antibody
    • POU domain class 3 transcription factor 2 antibody
    • POU domain, class 3, transcription factor 2 antibody
    • Pou3f2 antibody
    • POUF 3 antibody
    • POUF3 antibody
    • Protein Brn 2 antibody
    see all

Images

  • Anti-Brn-2 antibody (ab137469) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 46 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded TOV-21G xenograft labelling Brn-2 with ab137469 antibody at a dilution of 1/500.

References

This product has been referenced in:
  • Srikanth P  et al. Shared effects of DISC1 disruption and elevated WNT signaling in human cerebral organoids. Transl Psychiatry 8:77 (2018). Read more (PubMed: 29643329) »
  • Srikanth P  et al. Convergence of independent DISC1 mutations on impaired neurite growth via decreased UNC5D expression. Transl Psychiatry 8:245 (2018). Read more (PubMed: 30410030) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Zebrafish Cell (Retina)
Permeabilization
Yes - 0.2% Triton-x 100
Specification
Retina
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 25 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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