Anti-Brn-2 antibody (ab139734)
Key features and details
- Rabbit polyclonal to Brn-2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Brn-2 antibody
See all Brn-2 primary antibodies -
Description
Rabbit polyclonal to Brn-2 -
Host species
Rabbit -
Specificity
At least two isoforms are known to exist. ab139734 will recognize both isoforms. ab139734 is predicted to not cross-react with other members of the POU domain class 3 family. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Mouse
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Immunogen
Synthetic peptide corresponding to Human Brn-2 (C terminal).
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Positive control
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General notes
Protein name formally labeled as POU3F2.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab139734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Human
|
| WB |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
|
|
| ICC/IF |
Use a concentration of 20 µg/ml.
|
| Notes |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa. |
|
ICC/IF
Use a concentration of 20 µg/ml. |
Target
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Function
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. -
Tissue specificity
Expressed specifically in the neuroectodermal cell lineage. -
Sequence similarities
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5454 Human
- Entrez Gene: 18992 Mouse
- Omim: 600494 Human
- SwissProt: P20265 Human
- SwissProt: P31360 Mouse
- Unigene: 182505 Human
- Unigene: 389520 Mouse
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Alternative names
- Brain 2 antibody
- Brain specific homeobox/POU domain protein 2 antibody
- Brain-2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody (ab139734)
Immunofluorescence of Brn-2 in Human brain tissue with ab139734 at 20 ug/mL.
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Western blot - Anti-Brn-2 antibody (ab139734)Lane 1 : Anti-Brn-2 antibody (ab139734) at 1 µg/ml
Lane 2 : Anti-Brn-2 antibody (ab139734) at 2 µg/ml
All lanes : 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 46 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab139734 has not yet been referenced specifically in any publications.