Anti-Brn-2 antibody (ab139734)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Brn-2 antibody
See all Brn-2 primary antibodies -
Description
Rabbit polyclonal to Brn-2 -
Host species
Rabbit -
Specificity
At least two isoforms are known to exist. ab139734 will recognize both isoforms. ab139734 is predicted to not cross-react with other members of the POU domain class 3 family. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human Brn-2 (C terminal).
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Positive control
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General notes
Protein name formally labeled as POU3F2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab139734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa. | |
| ICC/IF | Use a concentration of 20 µg/ml. |
Target
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Function
Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters. -
Tissue specificity
Expressed specifically in the neuroectodermal cell lineage. -
Sequence similarities
Belongs to the POU transcription factor family. Class-3 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5454 Human
- Entrez Gene: 18992 Mouse
- Entrez Gene: 29588 Rat
- Omim: 600494 Human
- SwissProt: P20265 Human
- SwissProt: P31360 Mouse
- SwissProt: P56222 Rat
- Unigene: 182505 Human
see all -
Alternative names
- Brain 2 antibody
- Brain specific homeobox/POU domain protein 2 antibody
- Brain-2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody (ab139734)Immunofluorescence of Brn-2 in Human brain tissue with ab139734 at 20 ug/mL.
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Western blot - Anti-Brn-2 antibody (ab139734)Lane 1 : Anti-Brn-2 antibody (ab139734) at 1 µg/ml
Lane 2 : Anti-Brn-2 antibody (ab139734) at 2 µg/ml
All lanes : 3T3 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 46 kDa
Datasheets and documents
References
ab139734 has not yet been referenced specifically in any publications.
