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Anti-Brn-2 antibody (ab139734)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody (ab139734)
  • Western blot - Anti-Brn-2 antibody (ab139734)

Key features and details

  • Rabbit polyclonal to Brn-2
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Brn-2 antibody
    See all Brn-2 primary antibodies
  • Description

    Rabbit polyclonal to Brn-2
  • Host species

    Rabbit
  • Specificity

    At least two isoforms are known to exist. ab139734 will recognize both isoforms. ab139734 is predicted to not cross-react with other members of the POU domain class 3 family.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide corresponding to Human Brn-2 (C terminal).

  • Positive control

    • Purchase matching WB positive control:Recombinant Human Brn-2 protein
    • 3T3 cell lysate
  • General notes

    Protein name formally labeled as POU3F2.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer

    pH: 7.2
    Preservative: 0.02% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Notch Pathway
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • POU
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Transcription Factors

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Brn-2 protein (ab134864)
  • Related Products

    • Recombinant Human Brn-2 protein (ab134864)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab139734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Mouse
Application Abreviews Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
ICC/IF
Use a concentration of 20 µg/ml.
Notes
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 46 kDa.
ICC/IF
Use a concentration of 20 µg/ml.

Target

  • Function

    Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
  • Tissue specificity

    Expressed specifically in the neuroectodermal cell lineage.
  • Sequence similarities

    Belongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P20265 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 5454 Human
    • Entrez Gene: 18992 Mouse
    • Omim: 600494 Human
    • SwissProt: P20265 Human
    • SwissProt: P31360 Mouse
    • Unigene: 182505 Human
    • Unigene: 389520 Mouse
    • Alternative names

      • Brain 2 antibody
      • Brain specific homeobox/POU domain protein 2 antibody
      • Brain-2 antibody
      • Brain-specific homeobox/POU domain protein 2 antibody
      • BRN 2 antibody
      • Brn-2 antibody
      • BRN2 antibody
      • N Oct 3 antibody
      • N OCT 3 Gene antibody
      • N Oct3 antibody
      • Nervous system specific octamer binding transcription factor antibody
      • Nervous system-specific octamer-binding transcription factor N-Oct-3 antibody
      • OCT 7 antibody
      • Oct-7 antibody
      • OCT7 antibody
      • Octamer Binding Transcription Factor 7 antibody
      • Octamer-binding protein 7 antibody
      • Octamer-binding transcription factor 7 antibody
      • OTF 7 antibody
      • OTF-7 antibody
      • OTF7 antibody
      • PO3F2_HUMAN antibody
      • POU class 3 homeobox 2, antibody
      • POU domain class 3 transcription factor 2 antibody
      • POU domain, class 3, transcription factor 2 antibody
      • Pou3f2 antibody
      • POUF 3 antibody
      • POUF3 antibody
      • Protein Brn 2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody (ab139734)
      Immunocytochemistry/ Immunofluorescence - Anti-Brn-2 antibody (ab139734)

      Immunofluorescence of Brn-2 in Human brain tissue with ab139734 at 20 ug/mL.

    • Western blot - Anti-Brn-2 antibody (ab139734)
      Western blot - Anti-Brn-2 antibody (ab139734)
      Lane 1 : Anti-Brn-2 antibody (ab139734) at 1 µg/ml
      Lane 2 : Anti-Brn-2 antibody (ab139734) at 2 µg/ml

      All lanes : 3T3 cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 46 kDa

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab139734? Please let us know so that we can cite the reference in this datasheet.

    ab139734 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunocytochemistry abreview for Anti-Brn-2 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (human derived cortical neurons)
    Permeabilization
    Yes - 0.2% Triton
    Specification
    human derived cortical neurons
    Blocking step
    normal goat serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Dec 30 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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