• Product name
  • Description
    Rabbit polyclonal to Brn-2
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Human Brn-2 aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab106418)

  • Positive control
  • General notes

    Protein name formally labeled as POU3F2.



Our Abpromise guarantee covers the use of ab94977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
ICC/IF Use at an assay dependent concentration.


  • Function
    Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters.
  • Tissue specificity
    Expressed specifically in the neuroectodermal cell lineage.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-3 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain 2 antibody
    • Brain specific homeobox/POU domain protein 2 antibody
    • Brain-2 antibody
    • Brain-specific homeobox/POU domain protein 2 antibody
    • BRN 2 antibody
    • Brn-2 antibody
    • BRN2 antibody
    • N Oct 3 antibody
    • N OCT 3 Gene antibody
    • N Oct3 antibody
    • Nervous system specific octamer binding transcription factor antibody
    • Nervous system-specific octamer-binding transcription factor N-Oct-3 antibody
    • OCT 7 antibody
    • Oct-7 antibody
    • OCT7 antibody
    • Octamer Binding Transcription Factor 7 antibody
    • Octamer-binding protein 7 antibody
    • Octamer-binding transcription factor 7 antibody
    • OTF 7 antibody
    • OTF-7 antibody
    • OTF7 antibody
    • PO3F2_HUMAN antibody
    • POU class 3 homeobox 2, antibody
    • POU domain class 3 transcription factor 2 antibody
    • POU domain, class 3, transcription factor 2 antibody
    • Pou3f2 antibody
    • POUF 3 antibody
    • POUF3 antibody
    • Protein Brn 2 antibody
    see all


  • ab94977 staining Brn-2 Figure A: Human immortalized Schwann cells transfected with mouse Brn2, fixed and stained 2 days post transfection under conditions specified in above review. Figure B: Non-transfected control under the same staining conditions by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.3% in PBS for 10 mins at 21°C and blocked with 5% normal Goat serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/1000 in 5% Goat serum) for 22 hour at 4°C. An Alexa Fluor®488-conjugated Goat anti-Rabbit IgG polyclonal (1/500) was used as the secondary antibody for 2 hours at 25°C.

    See Abreview

  • All lanes : Anti-Brn-2 antibody (ab94977) at 1 µg/ml

    Lane 1 : E16 Mouse Embryo Brain Tissue Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 47 kDa
    Additional bands at: 200 kDa, 35 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes


This product has been referenced in:
  • Zhang Q  et al. Impaired Dendritic Development and Memory in Sorbs2 Knock-Out Mice. J Neurosci 36:2247-60 (2016). Read more (PubMed: 26888934) »
  • Brunjes PC & Osterberg SK Developmental Markers Expressed in Neocortical Layers Are Differentially Exhibited in Olfactory Cortex. PLoS One 10:e0138541 (2015). Read more (PubMed: 26407299) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Immunocytochemistry/ Immunofluorescence
Mouse Cell (cortical neurons)
cortical neurons
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 22 2016

Immunocytochemistry/ Immunofluorescence
Human Cell (Immortalized Schwann cells mouse Brn2 transfection)
Yes - 0.3% Triton X100 10 min at room temperature
Immortalized Schwann cells mouse Brn2 transfection
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 15 2012

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