Recombinant
RabMAb

Recombinant Anti-BRN3A antibody [EP1972Y] - BSA and Azide free (ab232480)

Overview

  • Product name
    Anti-BRN3A antibody [EP1972Y] - BSA and Azide free
    See all BRN3A primary antibodies
  • Description
    Rabbit monoclonal [EP1972Y] to BRN3A - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human BRN3A aa 300-400. The exact sequence is proprietary.
    Database link: Q01851

  • Positive control
    • Flow cyt: SH-SY5Y cells.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab232480 is a PBS-only buffer format of ab81213. Please refer to ab81213 for recommended dilutions, protocols, and image data.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.

Target

  • Function
    Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons.
  • Tissue specificity
    Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye.
  • Sequence similarities
    Belongs to the POU transcription factor family. Class-4 subfamily.
    Contains 1 homeobox DNA-binding domain.
    Contains 1 POU-specific domain.
  • Developmental stage
    Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Brain specific homeobox/POU domain protein 3A antibody
    • Brain-3A antibody
    • Brain-specific homeobox/POU domain protein 3A antibody
    • Brn 3.0 antibody
    • BRN 3A antibody
    • Brn-3A antibody
    • Brn3 antibody
    • BRN3A antibody
    • class 4 antibody
    • FLJ13449 antibody
    • Homeobox/POU domain protein RDC 1 antibody
    • Homeobox/POU domain protein RDC-1 antibody
    • Homeobox/POU domain protein RDC1 antibody
    • Oct T1 antibody
    • Oct-T1 antibody
    • OctT1 antibody
    • PO4F1_HUMAN antibody
    • POU class 4 homeobox 1 antibody
    • POU domain antibody
    • POU Domain Class 4 Transcription Factor 1 antibody
    • Pou4f1 antibody
    • RDC 1 antibody
    • RDC1 antibody
    • transcription factor 1 antibody
    see all

Images

  • Overlay histogram showing SH-SY5Y cells stained with unpurified ab81213 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81213, 1/50 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81213).

  • Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling BRN3A with purified ab81213 at 1:100 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor ® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81213).

References

ab232480 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab232480.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up