Recombinant Anti-BRN3A antibody [EPR23257-285] - BSA and Azide free (ab273099)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23257-285] to BRN3A - BSA and Azide free
- Suitable for: IHC-Fr, IP, Flow Cyt (Intra), WB, IHC-P
- Reacts with: Mouse, Rat, Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-BRN3A antibody [EPR23257-285] - BSA and Azide free
See all BRN3A primary antibodies -
Description
Rabbit monoclonal [EPR23257-285] to BRN3A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IP, Flow Cyt (Intra), WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant fragment -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: MOLT4 whole cell lysate. Mouse E14.5, mouse E18 and rat E18 brain tissue lysate. His-tagged Mouse POU4F1 recombinant protein. Flow Cyt (intra): MOLT4 cells. IHC-P: Mouse E14.5 cochleovestibular ganglion and colliculus. Rat E14.5 dorsal root ganglia. IHC-Fr: Mouse retina tissue. Mouse E14.5 embryo. IP: Mouse E14.5 brain tissue lysate.
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General notes
ab273099 is the carrier-free version of ab245230.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR23257-285 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab273099 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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IP |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 43 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
IP
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 42 kDa (predicted molecular weight: 43 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Probable transcription factor which may play a role in the regulation of specific gene expression within a subset of neuronal lineages. May play a role in determining or maintaining the identities of a small subset of visual system neurons. -
Tissue specificity
Brain. Seems to be specific to the retina. Present in the developing brain, spinal cord and eye. -
Sequence similarities
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Developmental stage
Expression peaks early in embryogenesis (day 13.5) and is undetectable 14 days after birth. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 5457 Human
- Entrez Gene: 18996 Mouse
- Entrez Gene: 114503 Rat
- Omim: 601632 Human
- SwissProt: Q01851 Human
- SwissProt: P17208 Mouse
- SwissProt: P20266 Rat
- Unigene: 654522 Human
see all -
Alternative names
- Brain specific homeobox/POU domain protein 3A antibody
- Brain-3A antibody
- Brain-specific homeobox/POU domain protein 3A antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling BRN3A with ab245230 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on cochleovestibular ganglion and colliculus of mouse E14.5 (PMID: 24709358). The section was incubated with ab245230 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245230).
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol-permeabilized Daudi (Human Burkitt's lymphoma lymphoblast (Left) / MOLT-4 (Human lymphoblastic leukemia T lymphoblast (Right) cells labelling BRN3A with ab245230 at 1/400 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor®488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control:Daudi (PMID: 20460523).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245230).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse retina tissue labeling BRN3A with ab245230 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on ganglion cell layer of mouse retina is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245230).
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BRN3A was immunoprecipitated from 0.35 mg mouse E14.5 brain tissue lysate with ab245230 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab245230 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse E14.5 brain tissue lysate 10µg.
Lane 2: ab245230 IP in mouse E14.5 brain tissue lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245230 in mouse E14.5 brain tissue lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This blot was developed using a higher sensitivity ECL substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245230).
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Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling BRN3A with ab245230 at 1/2000 dilution followed by ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on dorsal root ganglia of rat E14.5 (PMID: 8341591). The section was incubated with ab245230 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245230).
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 embryo tissue labeling BRN3A with ab245230 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Nuclear staining on mouse E14.5 embryonic brain is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab245230).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab273099 has not yet been referenced specifically in any publications.