Anti-BRN3B / POU4F2 antibody (ab166945)
Key features and details
- Goat polyclonal to BRN3B / POU4F2
- Suitable for: WB
- Isotype: IgG
Overview
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Product name
Anti-BRN3B / POU4F2 antibody
See all BRN3B / POU4F2 primary antibodies -
Description
Goat polyclonal to BRN3B / POU4F2This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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Host species
Goat -
Species reactivity
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Human, Pig, Chimpanzee, Zebrafish, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human BRN3B/ POU4F2 aa 239-252 (internal sequence).
Sequence:HGLPSHMGCMSDVD
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab166945 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
A 1 hour primary incubation is recommended for this product. Preliminary experiments gave an approx 70kDa band in Rat Retina lysates after 0.03µg/ml antibody staining.Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.2kDa according to NP_599182.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would welcome your feedback if you have tested this antibody in more suitable positive controls. |
Notes |
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WB
Use at an assay dependent concentration. A 1 hour primary incubation is recommended for this product. Preliminary experiments gave an approx 70kDa band in Rat Retina lysates after 0.03µg/ml antibody staining.Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 53.2kDa according to NP_599182.1. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would welcome your feedback if you have tested this antibody in more suitable positive controls. |
Target
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Function
Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. -
Tissue specificity
Brain. Seems to be specific to the retina. -
Sequence similarities
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 101132525 Chicken
- Entrez Gene: 428735 Chicken
- Entrez Gene: 471317 Chimpanzee
- Entrez Gene: 532453 Cow
- Entrez Gene: 482637 Dog
- Entrez Gene: 5458 Human
- Entrez Gene: 18997 Mouse
- Entrez Gene: 171355 Rat
see all -
Alternative names
- Brain specific homeobox/POU domain protein 3B antibody
- Brain-3B antibody
- Brain-specific homeobox/POU domain protein 3B antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab166945 has not yet been referenced specifically in any publications.